Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,

Solute Blue dextran Molecular Weight of solute Log' of the # Na2CrO4 2,000,000 Alkaline phosphatase Cytochrome C| 12,400 86,000 162 Log (Molecular wt.) 6.3 4.93 4.09 2.20 Elution Volume Ve (ml) 0.5M) AI 65 -6 2:3ML 3.5Ml 1. The total space surrounding the gel particles in the packed column, Vo = Ve of blue dextran. Why? V- 3.14 r²h Vt = Measure inner radius of the column, r = Measure the Height of the column bed, h= 2. Calculate the total volume occupied by the column Vt using following equation and data provided,

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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