3. You are loading a protein gel that holds up to 50ul. You need to load 25ug of protein sample that is at a concentration of 8 ug/ul. You are mixing your sample with a 4x Laemmli Buffer prior to loading. How much of each component will you mix and how much of total volume will you load in the well?

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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3. You are loading a protein gel that holds up to 50ul. You need to load 25ug of protein
sample that is at a concentration of 8 ug/ul. You are mixing your sample with a 4x
Laemmli Buffer prior to loading. How much of each component will you mix and how
much of total volume will you load in the well?
You-a-
EL
COL
DDOT.no:
Transcribed Image Text:3. You are loading a protein gel that holds up to 50ul. You need to load 25ug of protein sample that is at a concentration of 8 ug/ul. You are mixing your sample with a 4x Laemmli Buffer prior to loading. How much of each component will you mix and how much of total volume will you load in the well? You-a- EL COL DDOT.no:
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