Elution Buffer (50 ml) 20 mM HEPES 7.4 150 mM NaCl 250 mM imidazole

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter20: Applications Of Oxidation/reduction Titrations
Section: Chapter Questions
Problem 20.6QAP
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Calculate how much by volume of each concentrated stock solution you will need. Your starting materials are 1M HEPES 1 M midsole and 5 M salt. 

Elution Buffer (50 ml)
20 mM HEPES 7.4
150 mM NaCl
250 mM imidazole
Transcribed Image Text:Elution Buffer (50 ml) 20 mM HEPES 7.4 150 mM NaCl 250 mM imidazole
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