- * First, a stock solution of 10 mM fluorescein must be made in 15 mL of water. Calculate the mass of fluorescein you need to weigh out to make this solution. . Then, a dilution to 0.5 mM should be made in 15 mL of water. Calculate the volume of stock 10 mM fluorescein you need to add to make this solution. Then, the following dilutions should also be made in 10 mL each (using the 0.5 mM stock): . Fluorescein: 0.0005, 0.001, 0.0025, 0.005, 0.01, 0.05, and 0.1 mM. Calculate the volume of fluorescein stock and water needed to make these dilutions.
- * First, a stock solution of 10 mM fluorescein must be made in 15 mL of water. Calculate the mass of fluorescein you need to weigh out to make this solution. . Then, a dilution to 0.5 mM should be made in 15 mL of water. Calculate the volume of stock 10 mM fluorescein you need to add to make this solution. Then, the following dilutions should also be made in 10 mL each (using the 0.5 mM stock): . Fluorescein: 0.0005, 0.001, 0.0025, 0.005, 0.01, 0.05, and 0.1 mM. Calculate the volume of fluorescein stock and water needed to make these dilutions.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![First, a stock solution of 10 mM fluorescein must be made in 15 mL of
water. Calculate the mass of fluorescein you need to weigh out to make
this solution.
Then, a dilution to 0.5 mM should be made in 15 mL of water. Calculate
the volume of stock 10 mM fluorescein you need to add to make this
solution.
• Then, the following dilutions should also be made in 10 mL each (using
the 0.5 mM stock):
• Fluorescein: 0.0005, 0.001, 0.0025, 0.005, 0.01, 0.05, and 0.1 mM.
Calculate the volume of fluorescein stock and water needed to make these
dilutions.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9f650973-7173-4c31-9f1e-75b816bbf4f5%2F4f70b6de-fef7-48c3-b816-4d02456faa06%2F0wn2rxs_processed.jpeg&w=3840&q=75)
Transcribed Image Text:First, a stock solution of 10 mM fluorescein must be made in 15 mL of
water. Calculate the mass of fluorescein you need to weigh out to make
this solution.
Then, a dilution to 0.5 mM should be made in 15 mL of water. Calculate
the volume of stock 10 mM fluorescein you need to add to make this
solution.
• Then, the following dilutions should also be made in 10 mL each (using
the 0.5 mM stock):
• Fluorescein: 0.0005, 0.001, 0.0025, 0.005, 0.01, 0.05, and 0.1 mM.
Calculate the volume of fluorescein stock and water needed to make these
dilutions.
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