FIGURE 5-5 With the use of this method, Lederberg and Tatum demonstrated that genetic recombination between bacterial genotypes is possible. Mixing bacterial genotypes produces rare recombinants A в (a) The basic concept: two auxotrophic cultures (A- and B-) are mixed, yielding prototrophic wild types (WT). (b) Cells of type A- or type B- cannot grow on an unsupplemented (minimal) medium (MM) because A- andB- each carry mutations that cause the inability to synthesize constituents needed for cell growth. When Mix Some progeny WT (a) A- and B- are mixed for a few hours and A в met- bio- thr* leu* thi* Mixture met* bio* thr- leu- thi- then plated, however, a few colonies appear on the agar plate. These colonies derive from single cells in which genetic material has been exchanged; they are therefore capable of synthesizing all the required constituents of metabolism. Wash cells Wash cells Wash cells Plate - 108 cells Plate - 108 cells Plate - 108 cells MM MM MM No colonies met+ bio + thr+ leu+ thi+ Prototrophic colonies No colonies (b)
FIGURE 5-5 With the use of this method, Lederberg and Tatum demonstrated that genetic recombination between bacterial genotypes is possible. Mixing bacterial genotypes produces rare recombinants A в (a) The basic concept: two auxotrophic cultures (A- and B-) are mixed, yielding prototrophic wild types (WT). (b) Cells of type A- or type B- cannot grow on an unsupplemented (minimal) medium (MM) because A- andB- each carry mutations that cause the inability to synthesize constituents needed for cell growth. When Mix Some progeny WT (a) A- and B- are mixed for a few hours and A в met- bio- thr* leu* thi* Mixture met* bio* thr- leu- thi- then plated, however, a few colonies appear on the agar plate. These colonies derive from single cells in which genetic material has been exchanged; they are therefore capable of synthesizing all the required constituents of metabolism. Wash cells Wash cells Wash cells Plate - 108 cells Plate - 108 cells Plate - 108 cells MM MM MM No colonies met+ bio + thr+ leu+ thi+ Prototrophic colonies No colonies (b)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
In Figure 5-5,
a. Why do A− and B− cells, by themselves, not form
colonies on the plating medium?
b. What genetic event do the purple colonies in the
middle plate represent?
Transcribed Image Text:FIGURE 5-5 With the use of this
method, Lederberg and Tatum
demonstrated that genetic recombination
between bacterial genotypes is possible.
Mixing bacterial genotypes produces rare recombinants
A
в
(a) The basic concept: two auxotrophic
cultures (A- and B-) are mixed, yielding
prototrophic wild types (WT). (b) Cells of
type A- or type B- cannot grow on an
unsupplemented (minimal) medium (MM)
because A- andB- each carry mutations
that cause the inability to synthesize
constituents needed for cell growth. When
Mix
Some
progeny
WT
(a)
A- and B- are mixed for a few hours and
A
в
met- bio- thr* leu* thi*
Mixture
met* bio* thr- leu- thi-
then plated, however, a few colonies
appear on the agar plate. These colonies
derive from single cells in which genetic
material has been exchanged; they are
therefore capable of synthesizing all the
required constituents of metabolism.
Wash cells
Wash cells
Wash cells
Plate - 108 cells
Plate - 108 cells
Plate - 108 cells
MM
MM
MM
No
colonies
met+ bio + thr+ leu+ thi+
Prototrophic
colonies
No
colonies
(b)
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