Spread-plate method Solid agar Incubate Culture, diluted as needed Bacterial colonies 0.1-0.2 mL appear only on surface. Spread cells onto surface of pre-poured solid agar. Pour-plate method 0.1-1.0 mL Melted cooled agar Incubate FIGURE 4.18 Spread Plates and Pour Plates Some colonies appear on surface; many are below surface. These techniques can be used for plate counts. Add melted cooled agar and swirl gently to mix.
Spread-plate method Solid agar Incubate Culture, diluted as needed Bacterial colonies 0.1-0.2 mL appear only on surface. Spread cells onto surface of pre-poured solid agar. Pour-plate method 0.1-1.0 mL Melted cooled agar Incubate FIGURE 4.18 Spread Plates and Pour Plates Some colonies appear on surface; many are below surface. These techniques can be used for plate counts. Add melted cooled agar and swirl gently to mix.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells?
Transcribed Image Text:Spread-plate method
Solid agar
Incubate
Culture, diluted
as needed
Bacterial colonies
0.1-0.2 mL
appear only on surface.
Spread cells onto surface
of pre-poured solid agar.
Pour-plate method
0.1-1.0 mL
Melted cooled agar
Incubate
FIGURE 4.18 Spread Plates and Pour Plates
Some colonies appear on
surface; many are below surface.
These techniques can be used for plate counts.
Add melted cooled agar
and swirl gently to mix.
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