Figure 4 shows the results from Nanodrop quantification of DNA sample.Sarmple ID E Coli (K-12)Pedestal14ТурeDNA50 00Conc. 843.6ng/ulA260 (10 mm path)A280 (10 mm path)260 / 2802.11260 / 2302.24O Baseline correcton 340 nmWavelength (nm)Figure 4(i)Interpret the Nanodrop result above.(ii)How to improve the quality of the extracted DNA in order to obtain a better result?(ii)Calculate the absorbance of DNA at 260nm.10mm Absorbance

DNA is the genetic material in all the living organisms.

Answered: Figure 4 shows the results from…

Figure 4 shows the results from Nanodrop quantification of DNA sample. Sarmple ID E Col (K-12) Pedesta 14 Туре DNA • 50 00 12 Conc 843.6 ng/ul

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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☆回 w Insert Format Tools Add-ons Help Last edit was yesterday at 8:08 AM TURN 100% Normal text Arial 11 BIUA E- 1E E E - E EX 2. 3 . 5. C Directions: Describe the process of DNA replication in the space below. Specifically reference the pictures in your answer. Your response will undergo a plagiarism check. Be sure to write your response in your own words. Be sure to reference and thoroughly explain: The relationship and importance of DNA replication and the cell cycle. The conservation of genetic information during replication. (Is it conservative? Semi-conservation? Dispersive? What does this mean?) The specific enzymes used in each step and indicate whether each of the enzymes is a synthesis reaction or a digestion reaction. Justify your answer through explanation. Identify the parameters and limitations of the enzymes working effectively. (What factors would limit or interfere with DNA replication?)
Transcribe the following piece of DNA. DNA 5’ GCGATGCCCTAGGTATGA 3'
what analysis can be gathered from the raw count section and also what can also be said for the whole data as a whole. 500 in total please
Section B: Circular DNA Eco01091 2698 Pfol 46 BSTAPI 179 Aatll 2641 Sspl 2525 Ndel 183 Ehel 235 Pdml 2318 396 MCS lacz 146 Bcgl 2239 Scal 2201 \2510 236 476 493 PUC57 2710 bp BsaXI 683 Sapl 707 876, AfillI, Pscl 830 Gsul 1808 Cfr101 1803- Eco311 1790 Eam11051 1718 1650 1490 rep (PMB1) \BseYl 1134 \Cail 1241 te fenen P S GTAA AAC GAC Goc CA TGA ATT CGA GCT CGG TAC CTc cCG AAT GCA TCT AGA TAT CGG ATC ccG Gạc ccG TCG ACT GCA GAG GCC TGC ATG CAA GCT Too 3' CATT TO Cra cco orC ACT TAA OCT COA GCC ATG GAG cOC TTA COT AGA TCT ATA GCC TAG 0oc coa 00C AGC TGA COT CTC c00 ACO TACOTT COA Ace Lad Va V Al Le Se Aen Ser Se P Val C Arn e Cy Ag Ser e Pe A Arg Als Arg Arg Se O Le ay Al His Le Ser e caT AAT CAT Gar CAT AGC Tor Tre era 3 OCA TTA GTA CCA GTATOSACA AAG GAC S The le Met The Met 1. You have a circular plasmid (PUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes?. Show…
Gmetics pauben saiv Search (AtQ Stmder Pa d Design Transtians Ansmutions Shide Show Review View Resorang Help A A E E- E BIUS AY Aa P 4 Saes Arrange Slides Sdes Font Pargaph Sample Problems 1. A coin is flipped four times and comes up heads each tirme. What is the probability that the next coln flip will come up heads? Round (R) is dominant to wrinkled (r) Yellow (Y) is daminant to green(y) 2.Classify the following as heterozygous or homozygcus: RR, Rr, yy, YYRR 3. What is the phenotype of the following: Yy, RAr, y, YyRr 4 What is the probability of Rr x Rr producing a wrinkled seed? 5. What is the probability of Yyx yy producing green seeds? 6. What is the probability that Rry x RRYY would produce RrYy Click to add notes Daghs Senrgs 60'F Sunty 420
BamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…