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Figure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?

Figure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Experiment Title: Cytotoxicity Test Experiment no. 2 Objectives: Study the cytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Neerosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die look for an adequa compound/condition (cancer and immotherapy) 2. Or you want to exclude cyto icity in specific cells (chemicals and drugs) i have a report on this subject, please solve Applications - Drug discovery proces - Oncological research - Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, and my topic. industrial chemicals Classification of cytotoxicity and cell viability assays These essays are classified according to measurement types of endpoints (color changes, fluorescence, luminescent, etc.). 1. Dye exclusion: Trypan blue, cosin, Congo red, erythrosine B assays. 2. Colorimetric assays: MTT assay, MTS assay, XTT assay, WST-I assay. WST-8 assay, LDH assay, SRB assay, NRU assay, and crystal violet assay. 3. Fluorometric assays: alamarBlue assay and CFDA-AM assay. 4. Luminometric assays: ATP assay and real-time viability assay. 2- Experimental Procedure 1- Plate ceils into 96-well tissue culture plates. In general, 5000 - 10,000 ceils per well according to the cxperimental factors tested. Carry out your experiment by adding chemicals or biological agents into the appropriate well. Incubate for 6 to 24 hours. 2- remove 96 well plates from the incubator and add 20 ul MTT stock solution to each weil 3- place 96 well into an incubator at 37°C, 5% CO2 for 4 hours 4- remove 96 well plates fiom the incubator and aspirate the solution 5- add 100 ul DMSO to each well to dissolve the formazan 6- rotate the plate for 5 min to distribute eveniy 7- Record absorbance at 540nm Figure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?
Transcribed Image Text:Experiment Title: Cytotoxicity Test Experiment no. 2 Objectives: Study the cytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Neerosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die look for an adequa compound/condition (cancer and immotherapy) 2. Or you want to exclude cyto icity in specific cells (chemicals and drugs) i have a report on this subject, please solve Applications - Drug discovery proces - Oncological research - Safety evaluation of pesti ides, plantextracts, food additives, cosmetics, and my topic. industrial chemicals Classification of cytotoxicity and cell viability assays These essays are classified according to measurement types of endpoints (color changes, fluorescence, luminescent, etc.). 1. Dye exclusion: Trypan blue, cosin, Congo red, erythrosine B assays. 2. Colorimetric assays: MTT assay, MTS assay, XTT assay, WST-I assay. WST-8 assay, LDH assay, SRB assay, NRU assay, and crystal violet assay. 3. Fluorometric assays: alamarBlue assay and CFDA-AM assay. 4. Luminometric assays: ATP assay and real-time viability assay. 2- Experimental Procedure 1- Plate ceils into 96-well tissue culture plates. In general, 5000 - 10,000 ceils per well according to the cxperimental factors tested. Carry out your experiment by adding chemicals or biological agents into the appropriate well. Incubate for 6 to 24 hours. 2- remove 96 well plates from the incubator and add 20 ul MTT stock solution to each weil 3- place 96 well into an incubator at 37°C, 5% CO2 for 4 hours 4- remove 96 well plates fiom the incubator and aspirate the solution 5- add 100 ul DMSO to each well to dissolve the formazan 6- rotate the plate for 5 min to distribute eveniy 7- Record absorbance at 540nm Figure 1. Cytotoxicity test on 96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?
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