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Explain the principle of MALDI-TOF mass spectrometry.
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- Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The symptoms of the test animal may vary from the symptoms seen in the O 2) patient. O 3) Inoculate a test animal with the isolated organism. 4) The organism must be isolated from the test animal in pure culture. 5) The organism isolated from the test animal must match the organism isolated from the patient.The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein. 1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein 1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography 1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatographyDescribe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.
- Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.briefly explain the principle behind this technique; gel-filtration chromatographyTo analyze a sample by mass spectrometry, the sample must consist of gas phase ions. a) Explain why it is difficult to make gas phase ions of proteins. b) Generally explain how electrospray ionization turns proteins in solution into gas phase ions.
- Compare and contrast the following protein characterization techniques in terms of the principles governing their functions. electrosrapy ionization mass spectrometry vs polyacrylamide gel electrophoresisDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionGive the principle involved in the ninhydrin test. What is its purpose?