Explain the molecular basis of this observed thermal property of BPTI relative to the native structure and function of the protein.
Explain the molecular basis of this observed thermal property of BPTI relative to the native structure and function of the protein.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Globular proteins with multiple disulfide bonds must be heated longer and at higher temperature to denature them. Bovine
pancreatic trypsin inhibitor (BPTI), having 58 amino acids in a single chain and 3 disulfide linkages, loses its catalytic activity when
heated at nearly 90°C for 5-10 minutes. Explain the molecular basis of this observed thermal property of BPTI relative to the native
structure and function of the protein.
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