Explain the medium used is either synthetic or complex medium for fermentation production. Identify the carbon source used for cellulase production and give TWO examples of mesoentrient elements.
Explain the medium used is either synthetic or complex medium for fermentation production. Identify the carbon source used for cellulase production and give TWO examples of mesoentrient elements.
Chapter10: Reconstitution Of Powdered Drugs
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Problem 2.4P
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Transcribed Image Text:Medium used for cellulase production contained (g/L): NANO3, 3; MgSO4-7H2O, 0.5;
KHPO4, 1; KCI, 0.5 and supplemented with 1 mL FeSO5-7H2O as trace element. The
medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus
spore suspension (approximately 2x10' spores/mL) to 50 mL of sterilized medium
containing 5 % (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at
35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization
before proceeding to statistical optimization.
Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme
activity of Aspergillus terreus
Concentration
Endoglucanase
activity (U mL')
Protein content
Specific activity (U
mg' protein)
(mg mL-)
Bagasse (%)
1
0.32
0.90
0.35
0.47
1.83
0.25
3
0.65
2.50
0.26
4
0.88
3.16
0.28
5
0.96
3.30
0.29
0.73
1.88
0.39
Yeast extract (g L)
2.0
1.40
2.17
0.65
2.5
1.90
1.82
1.04
3.0
2.47
2.04
1.21
3.5
1.99
2.70
0.74
4.0
1.67
2.68
0.63
4.5
1.51
3.10
0.48
5.0
1.35
3.76
0.35
5.5
1.18
3.20
0.37
6.0
074
2.52
0.29
Transcribed Image Text:Explain the medium used is either synthetic or complex medium for fermentation
production.
Identify the carbon source used for cellulase production and give TWO examples
of mesoentrient elements.
Based on Table 1, predict the optimization method used by the researchers with
justification.
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