Having identified a different level of a particular collagen type in the mutant C. elegans strains, you wish to determine the genetic basis of the mutant phenotype. This collagen type is produced by the conversion of one type of collagen to another through modification by the enzyme Collagen Transferase B (encoded by the gene cob-1). Previous studies have shown the transcription of the cob-1 gene to be negatively regulated by a transcription factor, Collagen Modification Repressor (encoded by the gene rpr-5). You extract mature mRNA from true breeding wildtype and mutant worms, and use Reverse Transcription Quantitative PCR (qPCR) to measure the level of transcription of both the cob- 1 and rpr-5 genes. These results are displayed in Figure S1 of your in-class data sheet. 40 Wildtype Mutant 30 20. 10. Figure S1. Levels of cob-1 and rpr-5 mRNA in true-breeding wildtype and mutant C. elegans strains, as determined by RT-qPCR. Q2. What observations can be made regarding the relative expression of Collagen Transferase B and the Collagen Modification Repressor in the wildtype and the mutant C. elegans strains. What is a potential explanation for this difference? (3-4 sentences) Relative expression units cob-1 rpr-5
Having identified a different level of a particular collagen type in the mutant C. elegans strains, you wish to determine the genetic basis of the mutant phenotype. This collagen type is produced by the conversion of one type of collagen to another through modification by the enzyme Collagen Transferase B (encoded by the gene cob-1). Previous studies have shown the transcription of the cob-1 gene to be negatively regulated by a transcription factor, Collagen Modification Repressor (encoded by the gene rpr-5). You extract mature mRNA from true breeding wildtype and mutant worms, and use Reverse Transcription Quantitative PCR (qPCR) to measure the level of transcription of both the cob- 1 and rpr-5 genes. These results are displayed in Figure S1 of your in-class data sheet. 40 Wildtype Mutant 30 20. 10. Figure S1. Levels of cob-1 and rpr-5 mRNA in true-breeding wildtype and mutant C. elegans strains, as determined by RT-qPCR. Q2. What observations can be made regarding the relative expression of Collagen Transferase B and the Collagen Modification Repressor in the wildtype and the mutant C. elegans strains. What is a potential explanation for this difference? (3-4 sentences) Relative expression units cob-1 rpr-5
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Having identified a different level of a particular collagen type in the mutant
C. elegans strains, you wish to determine the genetic basis of the mutant phenotype. This
collagen type is produced by the conversion of one type of collagen to another through
modification by the enzyme Collagen Transferase B (encoded by the gene cob-1). Previous
studies have shown the transcription of the cob-1 gene to be negatively regulated by a
transcription factor, Collagen Modification Repressor (encoded by the gene rpr-5). You
extract mature mRNA from true breeding wildtype and mutant worms, and use Reverse
Transcription Quantitative PCR (qPCR) to measure the level of transcription of both the cob-
1 and rpr-5 genes. These results are displayed in Figure S1 of your in-class data sheet.
40
Wildtype
Mutant
30
20.
10.
Figure S1. Levels of cob-1 and rpr-5 mRNA in true-breeding wildtype and mutant C. elegans strains,
as determined by RT-qPCR.
Q2. What observations can be made regarding the relative expression of Collagen
Transferase B and the Collagen Modification Repressor in the wildtype and the mutant
C. elegans strains. What is a potential explanation for this difference? (3-4 sentences)
Relative expression units
cob-1
rpr-5
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