Homework Help is Here – Start Your Trial Now!
Science
Biology
2 Make a drawing of the simple recombinant plasmid PAMP/PKAN containing only the 3755bp and 1875bp with the ampicillin and kanamycin resistant genes. Label fragment sizes, ORI (origin of replication, restriction sites, and location of the antibiotic resistance gene(s). Calculate the total number of bp for the recombinant molecule and label it on your drawing. Use Figure 15.1 for reference. location of fragments

2 Make a drawing of the simple recombinant plasmid PAMP/PKAN containing only the 3755bp and 1875bp with the ampicillin and kanamycin resistant genes. Label fragment sizes, ORI (origin of replication, restriction sites, and location of the antibiotic resistance gene(s). Calculate the total number of bp for the recombinant molecule and label it on your drawing. Use Figure 15.1 for reference. location of fragments

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
Pretty sure I answered this wrong. Please help
2 Make a drawing of the simple recombinant plasmid PAMP/PKAN containing only the 3755bp and 1875bp fragments with the ampicillin and kanamycin resistant genes. Label fragment sizes, ORI (origin of replication, location of restriction sites, and location of the antibiotic resistance gene(s). Calculate the total number of bp for the recombinant molecule and label it on your drawing. Use Figure 15.1 for reference. 3755bp ampl PAMP Ban HI site 784 bp Hindll site BamHI site 2332 bp origins of replication PKAN KanI' 1875 bp Hindilsite Make a drawing of the double recombinant plasmid containing all four fragments. Label fragment sizes, ORI (origin of replication, location of restriction sites, and location of the antibiotic resistance gene(s). Calculate the total OHS
Transcribed Image Text:2 Make a drawing of the simple recombinant plasmid PAMP/PKAN containing only the 3755bp and 1875bp fragments with the ampicillin and kanamycin resistant genes. Label fragment sizes, ORI (origin of replication, location of restriction sites, and location of the antibiotic resistance gene(s). Calculate the total number of bp for the recombinant molecule and label it on your drawing. Use Figure 15.1 for reference. 3755bp ampl PAMP Ban HI site 784 bp Hindll site BamHI site 2332 bp origins of replication PKAN KanI' 1875 bp Hindilsite Make a drawing of the double recombinant plasmid containing all four fragments. Label fragment sizes, ORI (origin of replication, location of restriction sites, and location of the antibiotic resistance gene(s). Calculate the total OHS
EXERCISE 15.2 Ligation of Antibiotic Resistant Plasmids In this week's laboratory, you will complete your study of genetic engineering (biotechnology) by constructing a recombinant DNA plasmid which contains genes for both ampicillin resistance and kanamycin resistance. In this laboratory, you will use your digested antibiotic plasmids (Figure 15.1) from last week's laboratory to recon- struct a recombinant plasmid which possesses both the ampicillin resistance and kanamycin resistance genes. You will begin by heating your restriction digests of pAMP and 156 amp' PAMP 4539 BP ORI amp 3755 BP Digest with BamHI and Hindill 784 BP PKAN to destroy BamHI and HindIII activity. A sample from each reaction will be mixed with DNA ligase plus ATP and incubated at room temperature. Complementary BamHI and HindIII "sticky ends" hydrogen-bond to align restriction fragments. Ligase catalyzes the formation of phosphodiester bonds that covalently link the DNA fragments to form stable recombinant DNA molecules. BamHI 2332 BP BamHI 1875 BP Hindill kan' 274 PKAN 4207 BP ORI kan' ERPRE Hindill FIGURE 15.1 Top: PAMP and PKAN plasmid maps showing locations of origins of replication (ORI) and genes for ampicillin (amp) and kanamycin (kan) resistance in black. Bottom: Restriction digests of PAMP and PKAN showing locations of restriction enzyme (BamHI and HindIII) cutting sites and resulting DNA fragments including numbers of DNA base pairs (BP). Exploring Biology in the Laboratory this tut alread- 2X lig 3 Spin do use. 4 Add the tube: Samples Tube Ligase (initiale 5 Do PKA elec 6 Sp ter Pro Cast 1
Transcribed Image Text:EXERCISE 15.2 Ligation of Antibiotic Resistant Plasmids In this week's laboratory, you will complete your study of genetic engineering (biotechnology) by constructing a recombinant DNA plasmid which contains genes for both ampicillin resistance and kanamycin resistance. In this laboratory, you will use your digested antibiotic plasmids (Figure 15.1) from last week's laboratory to recon- struct a recombinant plasmid which possesses both the ampicillin resistance and kanamycin resistance genes. You will begin by heating your restriction digests of pAMP and 156 amp' PAMP 4539 BP ORI amp 3755 BP Digest with BamHI and Hindill 784 BP PKAN to destroy BamHI and HindIII activity. A sample from each reaction will be mixed with DNA ligase plus ATP and incubated at room temperature. Complementary BamHI and HindIII "sticky ends" hydrogen-bond to align restriction fragments. Ligase catalyzes the formation of phosphodiester bonds that covalently link the DNA fragments to form stable recombinant DNA molecules. BamHI 2332 BP BamHI 1875 BP Hindill kan' 274 PKAN 4207 BP ORI kan' ERPRE Hindill FIGURE 15.1 Top: PAMP and PKAN plasmid maps showing locations of origins of replication (ORI) and genes for ampicillin (amp) and kanamycin (kan) resistance in black. Bottom: Restriction digests of PAMP and PKAN showing locations of restriction enzyme (BamHI and HindIII) cutting sites and resulting DNA fragments including numbers of DNA base pairs (BP). Exploring Biology in the Laboratory this tut alread- 2X lig 3 Spin do use. 4 Add the tube: Samples Tube Ligase (initiale 5 Do PKA elec 6 Sp ter Pro Cast 1
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Immune disorders
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS