Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5 and supplemented with 1 mL FeSO5-7H20 as trace element. The medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus spore suspension (approximately 2x10' spores/mL) to 50 mL of sterilized medium containing 5 % (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at 35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization before proceeding to statistical optimization. Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme activity of Aspergillus terreus Concentration Endoglucanase activity (U mL) Protein content Specific activity (U mg' protein) (mg mL1) Bagasse (%) 1 0.32 0.90 0.35 0.47 1.83 0.25 0.65 2.50 0.26 4 0.88 3.16 0.28 0.96 3.30 0.29 0.73 1.88 0.39 Yeast extract (g L) 2.0 1.40 2.17 0.65 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 1.90 1.82 1.04 2.47 2.04 1.21 1.99 2.70 0.74 1.67 2.68 0.63 1.51 3.10 0.48 1.35 3.76 0.35 1.18 3.20 0.37 074 2.52 0.29
Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5; K2HPO4, 1; KCl, 0.5 and supplemented with 1 mL FeSO5-7H20 as trace element. The medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus spore suspension (approximately 2x10' spores/mL) to 50 mL of sterilized medium containing 5 % (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at 35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization before proceeding to statistical optimization. Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme activity of Aspergillus terreus Concentration Endoglucanase activity (U mL) Protein content Specific activity (U mg' protein) (mg mL1) Bagasse (%) 1 0.32 0.90 0.35 0.47 1.83 0.25 0.65 2.50 0.26 4 0.88 3.16 0.28 0.96 3.30 0.29 0.73 1.88 0.39 Yeast extract (g L) 2.0 1.40 2.17 0.65 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 1.90 1.82 1.04 2.47 2.04 1.21 1.99 2.70 0.74 1.67 2.68 0.63 1.51 3.10 0.48 1.35 3.76 0.35 1.18 3.20 0.37 074 2.52 0.29
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4 7H2O, 0.5;
K2HPO4, 1; KCl, 0.5 and supplemented with 1 mL FeSO5-7H20 as trace element. The
medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus
spore suspension (approximately 2x10' spores/mL) to 50 mL of sterilized medium
containing 5 % (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at
35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization
before proceeding to statistical optimization.
Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme
activity of Aspergillus terreus
Concentration
Endoglucanase
activity (U mL)
Protein content
Specific activity (U
mg' protein)
(mg mL1)
Bagasse (%)
1
0.32
0.90
0.35
0.47
1.83
0.25
0.65
2.50
0.26
4
0.88
3.16
0.28
0.96
3.30
0.29
0.73
1.88
0.39
Yeast extract (g L)
2.0
1.40
2.17
0.65
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
1.90
1.82
1.04
2.47
2.04
1.21
1.99
2.70
0.74
1.67
2.68
0.63
1.51
3.10
0.48
1.35
3.76
0.35
1.18
3.20
0.37
074
2.52
0.29
Transcribed Image Text:Explain the medium used is either synthetic or complex medium for fermentation
production.
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