Explain how scientists could have identified the T toxin   2. Name one enzyme which would have been used during cloning at stage 2.

Human Anatomy & Physiology (11th Edition)
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1. Explain how scientists could have identified the T toxin

 

2. Name one enzyme which would have been used during cloning at stage 2.

2.
Bacillus thuringiensis (Bt) is a bacterium that lives naturally in the soil. Bt produces a toxic protein
known as T toxin which is poisonous to caterpillars and some other insects. Genetic engineers have
inserted the gene for the production of T toxin into tomato plants. An outline of the procedure
which they used is given below.
|Identify and clone
T toxin gene
transfer gene into callus
of tomato cells
DNA extracted
from Bacillus
thuringiensis
tomato plant containing
cells capable of
producing T toxin
grow cells which have
the T toxin gene into
tomato plants
Transcribed Image Text:2. Bacillus thuringiensis (Bt) is a bacterium that lives naturally in the soil. Bt produces a toxic protein known as T toxin which is poisonous to caterpillars and some other insects. Genetic engineers have inserted the gene for the production of T toxin into tomato plants. An outline of the procedure which they used is given below. |Identify and clone T toxin gene transfer gene into callus of tomato cells DNA extracted from Bacillus thuringiensis tomato plant containing cells capable of producing T toxin grow cells which have the T toxin gene into tomato plants
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