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EXPERIMENT #4: POLYMERASE CHAIN REACTION Purpose: Using PCR technique, amplify the region of interest, the BXPO07 locus (a polymorphic allele) from DNA samples collected at a crime scene. Determine the genotypes of the samples using gel electrophoresis to determine if there is a match collected at the crime scene to one of the suspects. Background In 1993 Kary Mullis won the Nobel Prize for the molecular biology technique that became known as PCR (polymerase chain reaction). PCR has had an impact on five main areas of biotechnology: gene mapping cloning, DNA sequencing, gene detection, and DNA profiling PCR is now used routinely in the medical field as a diagnostic tool in detecting specific DNA mutations that may cause genetic disease as well as in the court of law to identify any suspects on a molecular level as part of the criminal investigation. PCR produces exponentially large amounts of a specific piece of DNA from trace amounts of starting material known as the template, which can be from any form of double-stranded DNA such as from a drop of blood, a hair follicle, a cheek cell, or a piece of bone. In theory PCR may generate millions of copies of new DNA molecules from a single intact strand of template DNA. The PCR cycle requires three steps: Denaturation: melting double-stranded DNA template into single strands. Heat is used to melt apart- or denature-the double-stranded DNA template into two separate single strands. Native (double helix) Denatured (random coll) • Annealing: complementary DNA strand hybridization via DNA primers. Short sequences of DNA upstream (5' end) and downstream (3' end) of the target loci region of interest must first be determined. These areas are then used to make short pieces of DNA called primers or oligonucleotides-which are complementary to regions upstream and downstream of the target loci region.

EXPERIMENT #4: POLYMERASE CHAIN REACTION Purpose: Using PCR technique, amplify the region of interest, the BXPO07 locus (a polymorphic allele) from DNA samples collected at a crime scene. Determine the genotypes of the samples using gel electrophoresis to determine if there is a match collected at the crime scene to one of the suspects. Background In 1993 Kary Mullis won the Nobel Prize for the molecular biology technique that became known as PCR (polymerase chain reaction). PCR has had an impact on five main areas of biotechnology: gene mapping cloning, DNA sequencing, gene detection, and DNA profiling PCR is now used routinely in the medical field as a diagnostic tool in detecting specific DNA mutations that may cause genetic disease as well as in the court of law to identify any suspects on a molecular level as part of the criminal investigation. PCR produces exponentially large amounts of a specific piece of DNA from trace amounts of starting material known as the template, which can be from any form of double-stranded DNA such as from a drop of blood, a hair follicle, a cheek cell, or a piece of bone. In theory PCR may generate millions of copies of new DNA molecules from a single intact strand of template DNA. The PCR cycle requires three steps: Denaturation: melting double-stranded DNA template into single strands. Heat is used to melt apart- or denature-the double-stranded DNA template into two separate single strands. Native (double helix) Denatured (random coll) • Annealing: complementary DNA strand hybridization via DNA primers. Short sequences of DNA upstream (5' end) and downstream (3' end) of the target loci region of interest must first be determined. These areas are then used to make short pieces of DNA called primers or oligonucleotides-which are complementary to regions upstream and downstream of the target loci region.

Phlebotomy Essentials
Phlebotomy Essentials
6th Edition
ISBN:9781451194524
Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)
Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)
Chapter1: Phlebotomy: Past And Present And The Healthcare Setting
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EXPERIMENT #4: POLYMERASE CHAIN REACTION Purpose: Using PCR technique, amplify the region of interest, the BXPO07 locus (a polymorphic allele) from DNA samples collected at a crime scene, Determine the genotypes of the samples using gel electrophoress to determine if there is a match collected at the crime scene to one of the suspects. Background In 1993 Kary Mullis won the Nobel Prize for the molecular biology technique that became known as PCR (polymerase chain reaction). PCR has had an impact on five main areas of biotechnology: gene mapping cloning, DNA sequencing, gene detection, and DNA profiling. PCR is now used routinely in the medical field as a diagnostic tool in detecting specific DNA mutations that may cause genetic disease as well as in the court of law to identify any suspects on a molecular level as part of the criminal investigation. PCR produces exponentially large amounts of a specific piece of DNA from trace amounts of starting material known as the template, which can be from any form of double-stranded DNA such as from a drop of blood, a hair follicle, a cheek cell, or a piece of bone. In theory PCR may generate millions of copies of new DNA molecules from a single intact strand of template DNA. The PCR cycle requires three steps: Denaturation: melting double-stranded DNA template into single strands. Heat is used to melt apart- or denature-the double-stranded DNA template into two separate single strands. Native (double helix) Denatured (random col) Annealing: complementary DNA strand hybridization via DNA primers. Short sequences of DNA upstream (5' end) and downstream (3' end) of the target loci region of interest must first be determined. These areas are then used to make short pieces of DNA called primers or oligonucleotides-which are complementary to regions upstream and downstream of the target loci region.
Transcribed Image Text:EXPERIMENT #4: POLYMERASE CHAIN REACTION Purpose: Using PCR technique, amplify the region of interest, the BXPO07 locus (a polymorphic allele) from DNA samples collected at a crime scene, Determine the genotypes of the samples using gel electrophoress to determine if there is a match collected at the crime scene to one of the suspects. Background In 1993 Kary Mullis won the Nobel Prize for the molecular biology technique that became known as PCR (polymerase chain reaction). PCR has had an impact on five main areas of biotechnology: gene mapping cloning, DNA sequencing, gene detection, and DNA profiling. PCR is now used routinely in the medical field as a diagnostic tool in detecting specific DNA mutations that may cause genetic disease as well as in the court of law to identify any suspects on a molecular level as part of the criminal investigation. PCR produces exponentially large amounts of a specific piece of DNA from trace amounts of starting material known as the template, which can be from any form of double-stranded DNA such as from a drop of blood, a hair follicle, a cheek cell, or a piece of bone. In theory PCR may generate millions of copies of new DNA molecules from a single intact strand of template DNA. The PCR cycle requires three steps: Denaturation: melting double-stranded DNA template into single strands. Heat is used to melt apart- or denature-the double-stranded DNA template into two separate single strands. Native (double helix) Denatured (random col) Annealing: complementary DNA strand hybridization via DNA primers. Short sequences of DNA upstream (5' end) and downstream (3' end) of the target loci region of interest must first be determined. These areas are then used to make short pieces of DNA called primers or oligonucleotides-which are complementary to regions upstream and downstream of the target loci region.
Lane Samples Which BXPOO7 Genotype alleles are present? CS 7,3 B'B 3 A. 10,3 B0B 4. 5,2 BS,B2 5 C B,X 6. D 10,2 B0,B? According to the table suspects that can be excluded from the investigation based on their locus is B'D because none of their alleles matches with the alleles found in the crime scene DNA. Suspects that cannot be excluded are A and D because their crime scene DNA alleles match with alleles. This could mean they are involved in the crime. Conclusion:
Transcribed Image Text:Lane Samples Which BXPOO7 Genotype alleles are present? CS 7,3 B'B 3 A. 10,3 B0B 4. 5,2 BS,B2 5 C B,X 6. D 10,2 B0,B? According to the table suspects that can be excluded from the investigation based on their locus is B'D because none of their alleles matches with the alleles found in the crime scene DNA. Suspects that cannot be excluded are A and D because their crime scene DNA alleles match with alleles. This could mean they are involved in the crime. Conclusion:
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