Recall that while FOVS will change with changing objectives, the size of the organism itself does not change. Consider the next example.

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Recall that while FOVS will change with changing objectives, the size of the organism itself does
not change. Consider the next example.
Example #1
Polyp
Objective: 40x
Example #2
Polyp
blObjective: 10x
Example #3
Polyp
Objective: 4x
Field of view: 500 µm
Field of view: 2000 µm
Field of view: 5000 µm
Estimated # of
Estimated # of
Estimated # of
organisms that fit
organisms that fit
organisms that fit
across the FOV: 1
Approximate size of
organism:
500μm/ 1 = 500 μm
across the FOV: 4
across the FOV: 10
Approximate size of
organism:
2000µm/ 4 = 500 µm
Approximate size of
organism:
5000μm/ 10-500 μm
%3D
The three examples above are of the same jellyfish polyp, but at varying objectives.
What is the size of the polyp at the 40x objective?
10x?
4x?
Which objective visualizes the polyp best?
Which objective would be best used to estimate organism size with?
Transcribed Image Text:Recall that while FOVS will change with changing objectives, the size of the organism itself does not change. Consider the next example. Example #1 Polyp Objective: 40x Example #2 Polyp blObjective: 10x Example #3 Polyp Objective: 4x Field of view: 500 µm Field of view: 2000 µm Field of view: 5000 µm Estimated # of Estimated # of Estimated # of organisms that fit organisms that fit organisms that fit across the FOV: 1 Approximate size of organism: 500μm/ 1 = 500 μm across the FOV: 4 across the FOV: 10 Approximate size of organism: 2000µm/ 4 = 500 µm Approximate size of organism: 5000μm/ 10-500 μm %3D The three examples above are of the same jellyfish polyp, but at varying objectives. What is the size of the polyp at the 40x objective? 10x? 4x? Which objective visualizes the polyp best? Which objective would be best used to estimate organism size with?
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A microscope is an instrument which is used to visualise microscopic images which are otherwise not visible to the naked eyes.

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