Electrophoretic flow and electroosmotic flow (EOF) plays an important role in capillary electrophoresis (CE) for ions movement.Explain the differences between electrophoretic flow and EOF.b) Suggest two different approaches (other than reducing the voltage) to reduce the electroosmotic flow. c) Three water soluble vitamins: niacinamide (a neutral compound), riboflavin (a neutral compound) and thiamine (a cation) were separated by micellar electrokinetic capillary chromatography (MEKC) in 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulfate. The migration times were niacinamide (7.5 min), riboflavin (12.8 min) and thiamine (13.9 min) Explain why these vitamins were separated using MEKC. Explain why niacinamide and riboflavin (both are neutral compounds) have different migration times.(i) With the aid of a diagram, differentiate flow profile of electroosmotic flow (EOF) and laminar flow. (ii) Discuss the effect of both flows in term of peak resolution.

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Electrophoretic flow and electroosmotic flow (EOF) plays an important role in capillary electrophoresis (CE) for ions movement.

Explain the differences between electrophoretic flow and EOF.
b) Suggest two different approaches (other than reducing the voltage) to reduce the electroosmotic flow.

c) Three water soluble vitamins: niacinamide (a neutral compound), riboflavin (a neutral compound) and thiamine (a cation) were separated by micellar electrokinetic capillary chromatography (MEKC) in 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulfate. The migration times were niacinamide (7.5 min), riboflavin (12.8 min) and thiamine (13.9 min)

Explain why these vitamins were separated using MEKC.

Explain why niacinamide and riboflavin (both are neutral compounds) have different migration times.

(i) With the aid of a diagram, differentiate flow profile of electroosmotic flow (EOF) and laminar flow.

(ii) Discuss the effect of both flows in term of peak resolution.

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