Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX.
Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX.
Chapter28: Atomic Spectroscopy
Section: Chapter Questions
Problem 28.4QAP
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Question
Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia
tinctorum, commonly known as rose madder. Earth spotted the root extract on two
hydrophobic silica gel plates. He then placed each plate in separate developing chambers,
one containing solvent system WHITE and the other housing solvent system MIX. Earth
allowed the chromatograms to develop for around five minutes. Afterwards, he removed
the plates from the containers and placed them inside an iodine chamber. The
chromatograms resulting from the TLC experiment after visualization are illustrated as
follows. The distances travelled by each spot and by the solvents were measured and
recorded in the displayed table.
Transcribed Image Text:D
75.0 mm
71.5 mm
DISTANCE
SOLVENT
SPOTS
TRAVELLED FROM
D
SYSTEM
THE ORIGIN, mm
13.0
32.5
solvent
front
A
В
WHITE
41.0
D.
A
B.
66.5
10.5
В
27.5
MIX
origin.
39.0
A
D.
53.5
10.5 mm
8 mm
WHITE
MIX
0 mm.
O mm
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