A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 mlof the suspension to 225 ml 0.90% Nacl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% Nacl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDITS pling COUNTS 1st dilution 54; 61 TNTC TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 st nd Brd TNTC Too numerous to count %3D ustrate the dilution series used and label the final dilution of each dilution.

A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 mlof the suspension to 225 ml 0.90% Nacl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% Nacl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDITS pling COUNTS 1st dilution 54; 61 TNTC TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 st nd Brd TNTC Too numerous to count %3D ustrate the dilution series used and label the final dilution of each dilution.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.21QAP

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Question

1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was
sampled to determine the population of the culture, by transferring 25 mlof the
suspension to 225 ml 0.90% NaCI. Three consecutive dilutions were further made by
using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in
each of duplicate plates. The following table shows the results of the plating
method.
Sampling
COUNTS
1st dilution
54; 61
TNTC
2nd dilution
3; 7
TNCT
3rd dilution
0; 0
242; 233
TNTC
4th dilution
0, 0
28: 37
249;246
1st
2nd
3rd
TNTC
TNTC
* TNTC
Too numerous to count
a. Illustrate the dilution series used and label the final dilution of each dilution.

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