E22. The method of Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing (discussed in Chapter 12) produces mRNAs of different lengths from the same gene. The Northern blot shown here was made using a DNA probe that is complementary to the MRNA encoded by a particular gene. The mRNA in lanes 1 through 4 was isolated from different cell types, and equal amounts of total cellular MRNA were added to each lane. 2 3 4 Lane 1: MRNA isolated from nerve cells Lane 2: MRNA isolated from kidney cells Lane 3: MRNA isolated from spleen cells Lane 4: MRNA isolated from muscle cells Explain these results. | |
E22. The method of Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing (discussed in Chapter 12) produces mRNAs of different lengths from the same gene. The Northern blot shown here was made using a DNA probe that is complementary to the MRNA encoded by a particular gene. The mRNA in lanes 1 through 4 was isolated from different cell types, and equal amounts of total cellular MRNA were added to each lane. 2 3 4 Lane 1: MRNA isolated from nerve cells Lane 2: MRNA isolated from kidney cells Lane 3: MRNA isolated from spleen cells Lane 4: MRNA isolated from muscle cells Explain these results. | |
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
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Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 10QP: The pre-mRNA transcript and protein made by several mutant genes were examined. The results are...
Related questions
Question
![E22. The method of Northern blotting is used to determine the amount
and size of a particular RNA transcribed in a given cell type.
Alternative splicing (discussed in Chapter 12) produces mRNAs
of different lengths from the same gene. The Northern blot shown
here was made using a DNA probe that is complementary to the
MRNA encoded by a particular gene. The mRNA in lanes 1 through
4 was isolated from different cell types, and equal amounts of total
cellular MRNA were added to each lane.
2
3
4
Lane 1: MRNA isolated
from nerve cells
Lane 2: MRNA isolated
from kidney cells
Lane 3: MRNA isolated
from spleen cells
Lane 4: MRNA isolated
from muscle cells
Explain these results.
| |](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe5061c68-3831-43a8-8e98-b24d0a655730%2F5f03c380-de29-4d3f-9304-203286a71a69%2Fj0be3om.png&w=3840&q=75)
Transcribed Image Text:E22. The method of Northern blotting is used to determine the amount
and size of a particular RNA transcribed in a given cell type.
Alternative splicing (discussed in Chapter 12) produces mRNAs
of different lengths from the same gene. The Northern blot shown
here was made using a DNA probe that is complementary to the
MRNA encoded by a particular gene. The mRNA in lanes 1 through
4 was isolated from different cell types, and equal amounts of total
cellular MRNA were added to each lane.
2
3
4
Lane 1: MRNA isolated
from nerve cells
Lane 2: MRNA isolated
from kidney cells
Lane 3: MRNA isolated
from spleen cells
Lane 4: MRNA isolated
from muscle cells
Explain these results.
| |
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