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During nucleic acid hybridization, the probe is labelled O for DNA stability O to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification

During nucleic acid hybridization, the probe is labelled O for DNA stability O to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification
Transcribed Image Text:During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification
You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which sequencing technology be most appropriate to use practically and economically: O Microarray Sanger sequencing RNA sequencing Exon sequencing
Transcribed Image Text:You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which sequencing technology be most appropriate to use practically and economically: O Microarray Sanger sequencing RNA sequencing Exon sequencing
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