Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. Mix the contents of each circle with a disposable stirrer while spreading over the entire area 5. enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF
RHEUMATOID FACTOR
1. Bring the test reagents and samples to room temperature (Note 1).
2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing.
3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop
of positive control serum and 1 drop of negative control into two additional circles.
4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested.
5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area
enclosed by the ring. Use separate stirrers for each mixture.
6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note
2).
7. Observe immediately under a suitable light source for any degree of agglutination.
Transcribed Image Text:Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.
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