+ DNA Fragment with Human Gene Recombinant Plasmid Plasmid Vector Bacterial DNA Recombinant Plasmid Transformed Bacterial Cell Bacterial Cell Produces Human Protein Genes for medically important proteins can be cloned and inserted into bacteria resulting in recombinant bacterial DNA, as shown in the diagram below Why can bacteria recognize a human gene and then produce a human protein? O Bacterial cells and human cells contain the same kind of chromosomes Bacterial cells contain the same organelles as human cells O DNA replication in bacteria and humans is the same The basic components of DNA are the same in humans and bacteria 이

Q: Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors…

A: Ans: Recombinant DNA technology: The given process is the process of recombinant DNA technology in…

Q: Which statement is NOT the function of restriction enzyme?

A: Answer: It ligates the desired to gene to vector’s plasmid.

Q: How is bacterial DNA safe from digestion by Restriction Endonucleases?     The plasmid of the…

A: Question-  How is bacterial DNA safe from digestion by Restriction Endonucleases?   A) The…

Q: What would be the DNA sequence of the new strar template? O 5'TAC GGT CTG 3' O 5'AAA GCG AAU 3' O…

A: it is simple as A pairs with T and C pairs with G. So where there is C place G nd vice versa and…

Q: DNA polymerase I DNA polymerase II DNA ligase Primase RNA primer 5' Lagging strand 3' 3' Okazaki…

A: The process of copying of double stranded DNA by the application of several Enzymes and proteins…

Q: In bacterial cells, nucleotide excision repair involves which of the following proteins? DNA…

A: The principal method utilised by mammals to remove bulky DNA lesions such as those caused by UV…

Q: In the following diagram of a replication fork, primers are shown as thick black lines and newly…

A: The heterocatalytic process by which a new DNA stand is synthesized on a old DNA template is known…

Q: In the gel electrophoresis: the mutant would just run the same as the open circle DNA if it simply…

A: In gel electrophoresis, the behavior of mutant DNA in comparison to open circle DNA and the presence…

Q: pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can…

A: Plasmids are the extra chromosomal material which are present independently of  the main…

Q: The function of DNA polymerase III is to? Choose below. Add nucleotides to the 3' end of the primer…

A: Replication is the process of producing identical replica of DNA molecule from the original DNA…

Q: In the diagram shown below representing a replisome, the portion represented by the letter functions…

A: Ans. The method of DNA replication is a process in which double-strand DNA molecule copying in order…

Q: The competency of bacterial cells to take up plasmids from the environment can be enhanced by…

A: The question is asking us to identify the correct statement regarding the process of enhancing the…

Q: Define the following words: replication fork, leading strand, lagging strand, Okazaki fragments,…

A: DNA replication is a biological process that occurs in all living organisms and is the basis for…

Q: Which of the following DNA repair systems could be used to repair the damage caused by UV light…

A: Due to the exposure to the UV rays, the structure of DNA gets distorted causing bends which leads to…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: It takes 40 minutes to completely replicate the E. coli chromosome, even in an optimally nourished…

A: Bacteria grow rapidly under the favorable environment where all the necessary conditions for its…

Q: Im designing a restriction cloning experiment. My professor said that plasmid and insert DNA work…

A: It would have been simpler if the digested product had been visible at a lower point on the gel.…

Q: What polypeptide sequence does the inserted gene code for? (Review protein synthesis and write the…

A: The polypeptide chain coding for the chain of amino acids can be known by the process of…

Q: Makes a DNA copy from a piece of RNA [Choose ] Reverse Transcriptase

A: In the method of Reverse Transcription ( RNA dependent DNA polymerase) convert Ribo nucleic acid…

Q: Which one is correct about telomeres? Choose at least one correct answer TTAGGG sequence is…

A: The distinctive structures that are found at the ends of our chromosomes and  consist of the…

Q: E. coli is severely exposed to UV light and is DNA damaged, but it attempts to replicate its genome.…

A: U.V. Light -- Ultra Violet Light -- It is the shortest wavelength of Ultra violet light emitted  by…

Q: Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…

Q: Beadle and Tatum Experiment X rays Conidia from the cultures that fail to grow on minimal medium are…

A: Beadle and Tatum experiment... Beadle and Tatum started their experiment by creating mutants of the…

Q: Clearly diagram rolling circle replication. Add 5’ and 3’ and show the direction of replication with…

A: A type of unidirectional nucleic acid replication involves the rolling circle replication where the…

Q: Give typed full explanation  you made a recombinant plasmid (it is circular) that contains the…

A: Here, the correct answer is option 1) The plasmid will be cut once, making a linear DNA fragment.

Q: Why does the probe bind to a specific site on a chromosome?

A: Cell division is the process by which a cell divides into two daughter cells. During this process,…

Q: What is the natural purpose of restriction endonucleases in bacteria? O To digest the bacterial…

A: According to the question, we have to find out what is the natural purpose of restriction…

Q: please answer these questions for this image What is the source of the DNA? What is the target?…

A: The objective of the question is to understand the process of genetic engineering, specifically the…

Q: You transform tetracycline sensitive competent cells with a plasmid solution (the plasmid encodes…

A: Tetracyclines were discovered in the 1940s and exhibited activity against a wide range of…

Q: Transcription V [Choose ] Coagulase RNA polymerase Translation DNA polymerse Ribosome Reverse…

A: The central dogma of molecular biology suggests the flow of genetic information from DNA to rna to…

Q: During which step in the transformation protocol does the plasmid DNA move into the cell? O Heat…

A: During a procedure termed transformation, bacteria may pick up foreign DNA. In DNA cloning,…

Q: Bacteriophage T4 has a linear double-stranded DNA genome, yet mapping many mutations, as shown in…

A: T4 bacteriophage infects E.coli. It has a double stranded linear DNA genome. It undergoes a lytic…

Q: In Figure 5-2, in which of the four processes shown can acomplete bacterial genome be transferred…

A: Conjugation, transformation, and transduction are the types of horizontal gene transfer mechanisms…

Q: Antibiotic resistance genes are often found on R plasmids (see Natural Gene Transfer and Antibiotic…

A: The substances that kill bacteria are known as antibiotics. As the antibiotics are used regularly…

Q: E.coli is replicating its chromosome. If the template DNA sequence is: 3' AAA CGC GAT 5', what would…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: During the polymerase chain reaction, heat is used to   Question 26 options:   break covalent…

A: Polymerase chain reaction (PCR) is a technique that is used to produce copies of DNA. It generates…

Q: Replicate, transcribe and translate the DNA strand below. Be sure to label your 5’ and 3’ ends and…

A: DNA has two strand, one strand is actively used as a template in the transcription process, this is…

Q: In E. coli, the leading strand is primarily synthesized by:       DNA polymerase ε   DNA polymerase…

A: DNA replication is a process in which the DNA makes multiple copies of itself.  The cell must…

Q: our plasn igest tub

A: Introduction: A plasmid is a small, circular, double-stranded DNA molecule that is separate from a…

Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: You are analyzing the intracellular DNA intermediates formed during the replication of the…

A: The provided experimental results involve the analysis of DNA intermediates in wild-type and mutant…

Q: Which of the following statements is true of plasmids? A Agrobacteria use plasmids to induce tumors…

A: Options - 1, 2, 4, 5, 6  are true

Q: You have two cell cultures, each containing a different plasmid. The first plasmid is -5kbp long and…

A: Cell culture is a process in which cells are grown outside their natural environment under…

Q: You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both…

A: Since only one out of two samples has plasmid DNA. The sample with DNA of other forms can be…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.