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- WIDAL TEST Principle: The test depends on the ability of antibody in the patient’s serum to agglutinate the stained bacterial antigens. When this occurs, the aggregates become clearly visible to the naked eye. Materials: Test tube 75 x 12 mm Physiological saline (0.9%) Incubator or water bath Slides Procedure: A. Rapid Slide Test Semi-Quantitative Method 1. Using a graduated pipette add the following amounts of serum to consecutive circles on a slide for each dilution under test. 0.08 ml, 0.04 ml, 0.02 ml, 0.01 ml and 0.005 ml 2. Thoroughly resuspend the antigen and add a drop to the appropriate circle on the slide. 3. Mix the drops and spread to cover the entire test circle. 4. Gently and evenly, rock and rotate the test slide for 1 minute then examine the slide for agglutination. 5. Results obtained correspond to tube agglutination titer of 1:20, 1:40, 1:80, 1:160, 1:320 respectively. 6. It is advisable to confirm a slide…Tighnari is doing preliminary protein studies on redcrest, a vibrant crimson fruit found in the hot deserts of Sumeru. He extracted proteins in the given sample using phosphate buffer. The resulting crude protein extract was subjected to gel filtration chromatography and SDS-PAGE. GFC results: - Absorbance Peak A B Figure 1. Elution profiles of the mixture of protein standards (black) and crude protein extract (blue). Table 1. Components of the calibration mixture and their corresponding molecular weight. U خلفة X A Elution Volume, mL Identity B Bovine Serum Albumin B-lactoglobulinn Horse Myoglobin Crude Protein Extract Molecular Weight, Da 66000 36800 17600Briefly discuss 2 advantages and 2 disadvantages of using the paraffin embedding method for histological examination of tissues as opposed to the frozen technique
- General Electrophoresis Questions: 1. Compare and contrast the sample buffers for DNA and protein electrophoresis. 2. Compare and contrast the running buffers for DNA and protein electrophoresis.Subject : Environmental Microbiology Can u use the information given below to answer these 2 question 1.Provide an aim for this lab 2. Provide objectives DISCUSSION QUESTIONS What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope? What determines the resolving power of the lens system? What is the limit of resolution obtainable with the light microscope? How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria? What advantages does electron microscopy have over light microscopy? What are disadvantages of electron microscopy over light microscopy? #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choicGeneral Electrophoresis Questions: 1. Why do we usually use agarose for DNA and polyacrylamide for proteins?2. Compare and contrast the sample buffers for DNA and protein electrophoresis.3. Compare and contrast the running buffers for DNA and protein electrophoresis.
- uses of 1D and 2D polyacrylamide gel electrophoresis techniques.les/test/tq.php?testid=2804&strandid%3D&element%3D&difficulty=assessment&assignment_id%3D45196931&load_test%-D1&teacherPr B Brainly.com-For st.. O Instagram N Netflix a Escape Room (Und. USATestprep, LLC - E Google Docs Stu wrerhd.cells at rer rote due to the siiple diffsion of Save C) 01ann suan an sianua LUPInaPLiaipaih ep snan aun aAPa IM JaPAA active transport. Glucose molecules will move from the cells into the solution in the beaker D) through facilitated diffusion. When examining the major macromolecules in the cell, a student isolates two molecule types that are directly responsible for cellular energy. A comparison of which molecule types will be most likely to include the molecules primarily responsible for cellular energy? A) proteins and lipids B) carbohydrates and lipids proteins and carbohydrates D) carbohydrates and nucleic acids Proteins and polysaccharides are polymers. These polymers are formed by dehydration synthesis. Which statement correctly identifies a…WIDAL TEST Principle: The test depends on the ability of antibody in the patient’s serum to agglutinate the stained bacterial antigens. When this occurs, the aggregates become clearly visible to the naked eye. Task: Illustrate(draw) how the antigens aggregate in the Widal Test
- Agarose Gel Electrophoresis Questions: 1. What determines how much agarose you should use in your gel? 2. Why do you see only DNA on the gel, and not protein? 3. How do you know which end of the gel to place the comb? 4. Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.I’m kinda confused on what to study for the test and what questions are gonna be on there but this PowerPoint but I just want you to explain the microbiology screenshots to me in a way I can clearly understand, I need a tutor