Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
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Chapter1: The Human Body: An Orientation
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Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
control
10
50
100
500
Concentration
absorbance
- TO
Figure 1. Graph showinf Growth inhibitory effects of Temazolomide on HCT-116
cells. absorbance Cells were seeded at a density of 2.5 x 10^3 cells/well in 96-well
plates and incubated for 72 h
et2
Absorbance (450nm)
B.
Transcribed Image Text:1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 control 10 50 100 500 Concentration absorbance - TO Figure 1. Graph showinf Growth inhibitory effects of Temazolomide on HCT-116 cells. absorbance Cells were seeded at a density of 2.5 x 10^3 cells/well in 96-well plates and incubated for 72 h et2 Absorbance (450nm) B.
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