Consider the reaction catalyzed by PFK. In the presence of ATP, which of thefollowing will be expected? Select all that apply.The T-state is stabilizedThe rate of the reaction is enhanced.The initial velocity plot would show a shift in the curve to the rightThe quaternary structure of PFK is stabilized to promote efficient binding of substrate

Phosphofructokinase-1 (PFK-1) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl…

Answered: Consider the reaction catalyzed by PFK.…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Explain under what conditions "substrate concentration" is the limiting factor for enzymatic reactions? what is the limiting factor when substrate concentration is not the limiting factor?
Match the following catalytic strategies with their example. Place a Letter on the picture. There are only two examples given. CH2OH CH2OH CH2OH CH2OH он он OH OH + H20 OH OH OH но H. но но OH OH OH OH OH OH A. Oxidoreducatase B. Transferase C. Hydrolase D. Lyase NH3 E. Isomerase F. Ligase R-CH-COO "ooc-CH2-CH2-C-COO Amino acid a-Ketoglutarate
Which of the following statements is correct for the reaction catalyzed by chymotrypsin? ○ The substrate carbon atom bonded to Ser195 in the acyl enzyme intermediate has a tetrahedral geometry ○ The Ser195 hydroxyl oxygen atom is the catalytic nucleophile for the deacylation phase of the overall reaction mechanism ○ The oxyanion hole accelerates the reaction by tightly binding the substrate carbonyl oxygen at the peptide bond that is cleaved ○ Asp102 stabilizes the tautomeric state of the catalytic histidine ○ The first reaction product contains a proton derived from an active-site water molecule
Put these steps in the mechanism of chymotrypsin catalysis in order from first to last. Note that some references may not break out each of these steps individually, but all steps should be ordered. There will be eight steps.
Sketch a graph showing how the concentration of product P varies with time for chymotrypsin-catalysed hydrolysis using a substrate that displays both Michaelis-Menten kinetics and burst kinetics. On the same graph show the effect of doubling the enzyme concentration, keeping all other conditions the same.
GAP + Pi + NAD+ ⟹1,3-BPG + NADH (Reaction 1) 1,3-BPG + ADP ⟹ 3-PG + ATP (Reaction 2) Which of the following statements concerning the information above is true? Select all that apply. A.Reaction 2 is an example of substrate level phosphorylation B.These reactions are written in a direction that would indicate gluconeogenesis is occurring in the liver C.The linking of Reaction 1 and Reaction 2 by the intermediate 1,3-BPG is an example of coupling of reactions D.Reaction 1 shows a redox reaction where the carbon skeleton is oxidized to generate electrons for a soluble electron carrier E.These reactions are irreversible under cellular conditions
PLP can catalyze both \alpha, \beta elimination reactions and \beta, \gamma - elimination reactions. Propose a mechanism for the following PLP - catalyzed \beta, \gamma elimination
Activity of the light reactions (ETC) can be assayed using an artificial, reducible compound (e- acceptor) added to take the place of the terminal electron acceptor. The artificial e- acceptor used is called DCPIP, a blue dye that turns colorless when it accepts electrons. The reaction is: H2O + DCPIPoxidized (blue) --> O2 + DCPIPreduced (colorless) What molecule from the light reactions of photosynthesis does DCPIP compete with?
Here’s a kinetic model for some enzyme E acting on substrate S. Give the equation for the change in the concentration of free enzyme (E) with time.
The enzymatic activity of an enzyme with Kg = 2 mM that converts substrate S into product P is measured at an initial substrate concentration S, of 10 µM. After 5 min, the substrate concentration is halved. What is the rate constant k, the maximal velocity vmax and the concentration of product after 12 min?
Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.
Which of the following statements best explains why the activity of ATP-citrate lyase and malic enzyme are often paired together? The use of ATP by ATP-citrate lyase means that NADH needs to be regenerated cytosolically so that more ATP can be generated via shuttle systems and replace the ATP used by ATP-citrate lyase ATP-citrate lyase produces ATP and malic enzyme produces NADPH, both needed in the fatty acid biosynthetic pathway The activity of ATP-citrate lyase and malic enzyme never are correlated, they always function in different realms of metabolism The generation of acetyl CoA is a substrate for fatty acid synthesis in the cytosol and NADPH is generated as the anabolic electron carrier

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