Nó Chloroplasts 0.45 Chloroplasts in the 0.4 dark Chloroplasts in the light 0.35 0.3 10 15 Time (minutes) w would you interpret the above data? Results indicate that the herbicide: a. interferes with the Calvin cycle and prevents electron transport b. interferes with the light reactions and prevents electron transport c had no effect on photosynthesis d. increased the rate of photosynthesis e. may have inhibited photosynthesis but the controls indicate the chloroplasts were not reducing DCPIP therefore the experiment wa herbicide 20
Nó Chloroplasts 0.45 Chloroplasts in the 0.4 dark Chloroplasts in the light 0.35 0.3 10 15 Time (minutes) w would you interpret the above data? Results indicate that the herbicide: a. interferes with the Calvin cycle and prevents electron transport b. interferes with the light reactions and prevents electron transport c had no effect on photosynthesis d. increased the rate of photosynthesis e. may have inhibited photosynthesis but the controls indicate the chloroplasts were not reducing DCPIP therefore the experiment wa herbicide 20
Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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![**Activity of Light Reactions in Photosynthesis: DCPIP Assay**
In studying the activity of light reactions, we can use an artificial, reducible compound serving as an electron acceptor substitute. The compound used here is DCPIP, a blue dye that becomes colorless when it accepts electrons. The chemical reaction involved is:
\[
\text{H}_2\text{O} + \text{DCPIP}_{\text{oxidized (blue)}} \rightarrow \text{O}_2 + \text{DCPIP}_{\text{reduced (colorless)}}
\]
**Experiment Overview**
Using a spectrophotometer, we can detect changes in DCPIP color by measuring light intensity as a function of wavelength. When light passes through a solution, it may be absorbed or scattered, affecting the solution's color and opacity. DCPIP absorbs light when oxidized but not when reduced. The data below illustrates an experiment that investigates the impact of an herbicide on photosynthesis.
**Graph Interpretation**
- **Y-Axis:** Absorbance
- **X-Axis:** Time (minutes)
The graph includes three sample conditions:
- **Green Line:** No Chloroplasts
- **Purple Line:** Chloroplasts in the Dark
- **Red Line:** Chloroplasts in the Light
The red line shows decreasing absorbance over time, indicating DCPIP reduction in the presence of light. The green line remains constant, and the purple line shows little change, indicating minimal or no DCPIP reduction.
**Question**
How would you interpret the above data? Results indicate that the herbicide:
a. Interferes with the Calvin cycle and prevents electron transport
b. Interferes with the light reactions and prevents electron transport
c. Had no effect on photosynthesis
d. Increased the rate of photosynthesis
e. May have inhibited photosynthesis, but controls indicate chloroplasts were not reducing DCPIP, making it difficult to assess the herbicide's effect.
Choose the best answer to see the potential impact on photosynthesis.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F09df8362-de22-4850-b335-73eb42cc5693%2F27a44d25-cd07-4696-b2fb-0efb260942a1%2Fw8btb5k_processed.jpeg&w=3840&q=75)
Transcribed Image Text:**Activity of Light Reactions in Photosynthesis: DCPIP Assay**
In studying the activity of light reactions, we can use an artificial, reducible compound serving as an electron acceptor substitute. The compound used here is DCPIP, a blue dye that becomes colorless when it accepts electrons. The chemical reaction involved is:
\[
\text{H}_2\text{O} + \text{DCPIP}_{\text{oxidized (blue)}} \rightarrow \text{O}_2 + \text{DCPIP}_{\text{reduced (colorless)}}
\]
**Experiment Overview**
Using a spectrophotometer, we can detect changes in DCPIP color by measuring light intensity as a function of wavelength. When light passes through a solution, it may be absorbed or scattered, affecting the solution's color and opacity. DCPIP absorbs light when oxidized but not when reduced. The data below illustrates an experiment that investigates the impact of an herbicide on photosynthesis.
**Graph Interpretation**
- **Y-Axis:** Absorbance
- **X-Axis:** Time (minutes)
The graph includes three sample conditions:
- **Green Line:** No Chloroplasts
- **Purple Line:** Chloroplasts in the Dark
- **Red Line:** Chloroplasts in the Light
The red line shows decreasing absorbance over time, indicating DCPIP reduction in the presence of light. The green line remains constant, and the purple line shows little change, indicating minimal or no DCPIP reduction.
**Question**
How would you interpret the above data? Results indicate that the herbicide:
a. Interferes with the Calvin cycle and prevents electron transport
b. Interferes with the light reactions and prevents electron transport
c. Had no effect on photosynthesis
d. Increased the rate of photosynthesis
e. May have inhibited photosynthesis, but controls indicate chloroplasts were not reducing DCPIP, making it difficult to assess the herbicide's effect.
Choose the best answer to see the potential impact on photosynthesis.
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