CONCENTRATION (NG/ ML) ABSORBANCE 655 NM (AU) 0.695 0.699 1000 500 250 0.587 125 0.513 63 0.385 31 0.294 16 О.18 8 0.119 0.089 4 0.077 1 0.078 0.068 AVG ABSORBANCE (nm) Concentration (ng/mL) Positive Control 0.699 0.698 0.697 0.699 Negative Control 0.081 0.083 0.088 0.08 Unknown #1 0.667 0.633 0.676 0.606 Unknown #2 0.06 0.173 0.064 0.053
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
The first table is known, and the second is unknown. we are trying to determine by ELISA if unknown samples (1 & 2) contain antibodies for chicken gamma globulin antigen, which sample contain the antigen?
•Create Fig 1 -Draw a standard curve using the semilog graph paper. Remember, your X-axis is your absorbance at 655nm (linear scale), and your Y-axis is your concentration in ng/ml (log scale)
•Show the concentrations of unknowns (how you find them by drawing vertical lines) in Fig 1.
Please label the negative and positive control and the unkown 1 and 2.
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Which Uknown 1 or 2, have antibodies for chicken gamma globulin antigen, also can you label the unknown 1 and 2 and the negative and positive control on the graph, thank you.
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