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Discuss the applications of molecular diagnostic tests
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- hello sir xplain why it is important to evaluate or measure blood compatibility with biomaterial???present briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imaging
- Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. What to do? Calculating the average absorbance of the standards Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.Bradford Assay Fill in the average A595 and A595 of sample minus A595 of blank. Show some sample computation. Concentration (micrograms/ml) A595 - trial 1 A595 - trial 2 A595 - trial 3 average A595 A595 of sample minus A595 of blank 0 1.501 1.446 1.447 2 1.624 1.558 1.559 5 1.731 1.749 1.712 10 1.901 1.838 1.892 15 2.161 2.108 2.228 18 2.231 2.277 2.319
- Please asap. thankyou. The following diagram represents the results of hemoglobin samples of 5 patients (1-5) that were run on an electrophoresis gel at pH 9.2. The first 3 lanes show the control samples, for comparison. Using only the letters "AA" "AS" and "SS", fill in the blanks below indicating the most likely genotype of each patient, based on these protein electrophoresis results. The following diagram represents the results of hemoglobin samples of 5 patients (1-5) that were run on an electrophoresis gel at pH 9.2. The first 3 lanes show the control samples, for comparison. Using only the letters "AA" "AS" and "SS", fill in the blanks below indicating the most likely genotype of each patient, based on these protein electrophoresis results. Patient 1 Patient 2 Patient 3 Patient 4 Patient 5Explain the advantages of using cell based assays for drug screening? Pls no chat gptGraph SEQ Graph ARABIC I p24 po/ml 10 7. 3) Quantitative skills: Using the graph below, determine the approximate concentration of p24 capsid protein (in kg/L) after 6 days of transfection with the 168.1/R molecular clone. 188.10 10 188.VR Include your calculations within the following space: 168.1 108.VT 10 10 10 10 10 12 14 days after transfection