Compute for the AST (Aspartate Aminotransferase activity) of 0.10 mL serum sample mixed with 1mL of reagent which yielded a A Abs/min reading of 0.003 at 340 nm with a molar absorptivity of 0.00622.
Q: Compute for the albumin to globulin ( A/G) ratio using these data given Albumin =51.9 g/L Total…
A: Different macromolecules are present in the body. They include carbohydrates, proteins, nucleic…
Q: Calculate the Activity of an amylase enzyme which is diluted 1:100 times with phosphate buffer and…
A: Given Values in the question are as follows: Dilution factor = 100 Incubation time = 10 min at 37°C…
Q: Assume that the experiments performed in the absence of inhibitors were conducted by adding 5 μL of…
A: We are asked to find some enzyme kinetics parameter that has a unit of s-1 . And in order to find…
Q: The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the…
A: Introduction Electrophoresis is a technique used to separate and analyze charged particles, such as…
Q: columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in…
A: Michaelis Menton equation relates velocity of enzymatic reaction with substrate…
Q: From this standard curve and chart below, does the separation of molecules in the mixture appear…
A: Size exclusion chromatography is a technique used to separate molecules by the virtue of their…
Q: Protein concentration can readily be determined using the Beer-Lambert law: A = e l c where A =…
A: According to Beer-Lambert law: A = e l c where A = absorbance e = molar absorption coefficient…
Q: suctose density gradient ultractifugation is a powerful technique for fractionating macromolecules…
A: The sucrose density gradient is used for fractionating the DNA, RNA, and protein. The samples are…
Q: After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is…
A: The rate of reaction is the rate of change of concentration of a reactant or product with time, then…
Q: Based on the experimental data provided, estimate the amount of amino acid in the given unknown…
A: Background information Ninhydrin is a strong oxidizing agent. It causes oxidative decarboxylation…
Q: This graph represents a standard curve from a BCA assay for protein concentration. If your BCA…
A: Standard curve can be constructed by using the known concentrations of a sample and their…
Q: Discuss the limitations of the lineweaver-burk plot and suggest alternative secondary plots and…
A: An enzyme is a biocatalyst that increases the rate of chemical reaction without itself being changed…
Q: q41 please calculate the unknown concentration of the protein D wih an absorbance value of A412…
A: A linear regression analysis will be required to determine the unknown concentration of Protein D.…
Q: Determine a molecular cause that allows explaining the BIOCHEMICAL alterations found in the…
A: a total cholesterol level of less than 200 mg/dL (5.2 mmol/L) is considered desirable and associated…
Q: A protein required 6.8 min to travel 82 cm to the detector in a 96 cm -long capillary tube with 25.4…
A: Introduction Using the formula, μ=ν×E-1Also, E= VLμ=ν×LVwhere μ is the electrophoretic mobility; ν…
Q: Neutral sphingomyelinase 2 converts sphingomyelin into ceramide and phosphorcholine. What kind of…
A: Neutral sphingomyelinase 2 (nSMase2) helps in breakdown of sphingomyelin to ceramide and…
Q: Does this calculation look correct? My goal isto have a target mass of 10 µg of my protein with a…
A: Yes this calculations look correct.since you want your final volume to be 30 microlitres you have to…
Q: つく 146 Bb 9146 Bb 10311 Class E Etsy Traps E Traps New Free Chat + ☆ 口出…
A: Turnover number refers to the number of substrate molecules that is converted to the product…
Q: Determine the expiration date of a drug product with the initial drug concentration of 50 mg/tablet…
A: A medication is a drug that is used to identify, treat, or prevent disease. It is also referred to…
Q: Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280…
A: Monoclonal antibodies are laboratory-produced molecules that are engineered to mimic the immune…
Q: Various concentrations of recombinant human insulin were prepared for use standards for an HPLC…
A: Beer's Law states that the absorbance is proportional to concentration of the given solute which is…
Q: The A280 of a protein sample loaded onto a gel was determined to be 0.767 (1.00 cm path length,…
A: Measuring protein concentration directly from absorbance is a simple and faster way to quantify…
Q: Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations…
A: The Bradford protein assay is a quick and accurate spectroscopic analytical procedure used to…
Q: What are the advantages of gel filtration as a technique for protein purification? Identify two…
A: The process of segregation of mixture is called chromatography using mobile and stationary phases.…
Q: calculate the volume of stock solutions required to make up the buffer solutions that will be used…
A: Introduction: A buffer solution is a solution that changes pH slightly with the addition of a strong…
Q: (a) Plot the data in the form of log Mr versus elution volume. From the line of best fit through…
A: Given data: Protein Mr Vel(mL) Blue dextran 106 85 Lysozyme 14,000 200 Chymotrypsin…
Q: Calculate the concentration of p-aminophenol control to match the 0.075 % w/w limit in 200 mg/10 ml…
A: Given : 0.075% w/w limit in 200 mg/10 mL Asked : Concentration calculation.
Q: From a kinetics experiment, kcat was determined to be 295sec-1. For the kinetic assay, 0.3mL of a…
A: Let the initial concentration of the enzyme in the reaction mixture be E0E0 = (Volume of enzyme…
Q: The purification continues with a cation exchange step in which the positively charged cytochrome C…
A: A linear XY linear graph for the equation. A straight line "y = mx + b" where y = absorbance at 595…
Q: Explain the Rf value obtained on the basis of the structure of amino acids use. Explain in 1-3…
A: The Rf value, also known as the retention factor, is a measure of the position of a component in a…
Q: 0.9% (m/v) NaCI solution and 5% (m/v) glucose solution are both isotonic to red blood cells. SHOW…
A: Osmolarity is the number of solutes present per litre of the solution. Tonicity is the capability…
![5. Compute for the AST (Aspartate
Aminotransferase activity) of 0.10 mL serum
sample mixed with 1mL of reagent which
yielded a A Abs/min reading of 0.003 at 340
nm with a molar absorptivity of 0.00622.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb9e84ed1-dead-4e59-995d-c6d6a75232f6%2F4b385fbf-c369-4d77-ad1c-48b41bbdece7%2F9nfrrb_processed.png&w=3840&q=75)
![](/static/compass_v2/shared-icons/check-mark.png)
Step by step
Solved in 3 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
- One general guideline for the maintenance dosing of heparin in pediatric patients is 100 units/kg every4 hours, or 20,000 units/m²/24 hour administered continuously. The a vailable injection for use by intravenous infusion contains 1000 USP Heparin Units/mL For a 44-lb child, measuring 42 inches in height, calculate the difference between the quantities of heparin administered over a 24-hour period in (a) heparin units, (b) in milligrams of heparin (sod ium), and (c) in milliliters of heparin injection.Calculate the oral loading dose (LD) of digoxin for Miss Jones who weighs 60 kg. The desired plasma concentration (Cp) is 1.5 mcg/L, volume of distribution (Vd) is 7.3 L/kg, the salt factor (S) is 1 and bioavailability fraction (F) is 0.7.Give your answer to the nearest whole number of micrograms.The equation below can be used:Loading Dose = Cp desired x VdS x F65. In acute hypersensitivity reactions, 0.5 mL of a 1:1000 (w/v) solution of epi- nephrine may be administered subcuta- neously or intramuscularly. Calculate the milligrams of epinephrine given. 65. 0.5 mg epinephrine
- a 14years client with type 1 diabetes weighing 110 pounds has an attack of hypoglycemia. he has been prescribed 0.5 g/kg of 50% dextrose. the 50% dextrose is supplied in 0.5 mg/mi prefilled syringes calculate thr dosage for the client by converting the weight into kilograms and the amount of dextrose in the syringe into gram. solution- 1.5ml (show work) 110lbs- 49.8 kgPaclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL X
- The order in the MAR is for Atropine 15 mg IV q6h hours PRN. How many mL will you give if the dosage strength is 75mg/7.5mL?Erythromycin lactobionate 500 mg Dexamethasone Na phosphate 100 mg Glycerin 2.5 mL Sterile water for injection ad 100 mL M. ft. opthalmic solution a. what is the percentage strength of erythromycin lactobionate in the prescription? b. If glycerin has a specific gravity of 1.25, what is its concentration (in w/v) in the prescription?For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.
- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.Your 64YO 152lb, 5ft 8in female patient daignosed with advanced bladder cancerhas been prescribed ifosamide 1500mg/m2/day IV for 5 days. You calculate the BSA using the Mosteller method as being 1.82m2. Ifosfamide is available in 60mL vials containing 3g of drugs. How many mL will you administer each day?What is the approximate rate of change of A340 measured? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 30 ml of the ADH solution was tested in the same way (i.e. half the amount of protein) ? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 60 ml of a 0.5 μM ADH solution was tested in the same way? i.e. ΔA340 / min = __________ As well as writing your answers, explain your reasoning.