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Compare the experimentally defined locations of the amino acids from the SCAM data with the positions predicted in the topological model. Comment on whether these experimental data are consistent with the topology model; do the data support this model? If not, how do they differ?

Compare the experimentally defined locations of the amino acids from the SCAM data with the positions predicted in the topological model. Comment on whether these experimental data are consistent with the topology model; do the data support this model? If not, how do they differ?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Compare the experimentally defined locations of the amino acids from the SCAM data with the positions predicted in the topological model.

Comment on whether these experimental data are consistent with the topology model; do the data support this model? If not, how do they differ?

kDa 55 Cysless 35 20 10 F16C E39C L860 G125C + + +U M18C V51C Y96C F139C M36C K72C ---+ MTSES + - NEM + + + MPEG5K |D106C +P ←U R143C +P ←U + MTSES - NEM + MPEG5K +P +U + MTSES - NEM +++ MPEG5K +P ←U + MTSES NEM +++ MPEG5K Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
Transcribed Image Text:kDa 55 Cysless 35 20 10 F16C E39C L860 G125C + + +U M18C V51C Y96C F139C M36C K72C ---+ MTSES + - NEM + + + MPEG5K |D106C +P ←U R143C +P ←U + MTSES - NEM + MPEG5K +P +U + MTSES - NEM +++ MPEG5K +P ←U + MTSES NEM +++ MPEG5K Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
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Transcribed Image Text:CO w
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