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Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached. Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.

Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached. Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached.

Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.

Score 3.5 3 2.5 2 1.5 1 0.5 0 -0.5 -1 -1.5 20 ProtScale output for user_sequence 40 Hydropath. / Kyte, & Doolittle 60 80 Position 100 120 140
Transcribed Image Text:Score 3.5 3 2.5 2 1.5 1 0.5 0 -0.5 -1 -1.5 20 ProtScale output for user_sequence 40 Hydropath. / Kyte, & Doolittle 60 80 Position 100 120 140
Predicted topologies and predicted AG values: TOPCONS OCTOPUS Philius PolyPhobius SCAMPI SPOCTOPUS PDB-homology ***No homologous TM proteins detected*** TOPCONS - Inside - Outside TM-helix (IN->OUT) TM-helix (OUT->IN) Signal peptide Consensus prediction (TOPCONS): Reliability 0.9 20 20 40 40 60 60 80 80 Position 100 100 120 120 140 10 140 8 6 2 0 AG (kcal/mol) High-resolution image High-resolution image
Transcribed Image Text:Predicted topologies and predicted AG values: TOPCONS OCTOPUS Philius PolyPhobius SCAMPI SPOCTOPUS PDB-homology ***No homologous TM proteins detected*** TOPCONS - Inside - Outside TM-helix (IN->OUT) TM-helix (OUT->IN) Signal peptide Consensus prediction (TOPCONS): Reliability 0.9 20 20 40 40 60 60 80 80 Position 100 100 120 120 140 10 140 8 6 2 0 AG (kcal/mol) High-resolution image High-resolution image
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