Choose all that are correct regarding impeller type choice in bioreactors.O Marine type impellers are ideal for shear sensitive mammalian cells.O Marine type impellers are ideal for less shear sensitive yeast cells.O One function of the impeller is to mix the contents of the bioactor (e.g., nutirents).O None of these options are true.O Impeller type choice depends on the shear sensitivity of the target cells and the solution viscosity.Choose all that are true regarding the phases of cell growth in a bioreactor.O None of these options are true.O There will always be a death phase in the bioreactor, since some cells are dying throughout.O Higher variability in the bioreactor contents is associated with stationary phase.O Higher varaibility in the bioreactor contents is associated with exponential phase when the cells are growingmost rapidly.O There will always be a lag phase of growth in a bioreactor since the cells need time to adjust to their newenviroment upon innoculation.Choose all of the correct statements regarding harvesting from a bioreactor.O Harvesting occurs after downstream processing of the product.O None of these options are true.O The harvest time will ideally occur when cells have changed their metabolism.O The harvest time will ideally occur in late stationary phase for growth-associated products.O The harvest time will ideally occur in stationary phase just before reaching the dealth phase and the cellconcentration begins to decrease.

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Downstream processing will primarily be concerned with initial separation of the bioreactor broth into a liquid phase and a solid phase, and subsequent concentration and purification of the product. Explain this statement
An industrial waste contains an inhibitory compound for microorganisms. You have determined that biological treatment would be the best process option if this inhibitory compound was not present. Identify and discuss your approach for handling this problem.
A continuous enzyme reactor consists of a stirred tank of volume 0.01 m3 through which liquid is pumped at 0.02 m3⁄h. The enzyme is immobilised onto non-porous, spherical support particles, and the contents are stirred well to ensure that the composition is uniform and that the particles remain in suspension. A plastic mesh has been placed in front of the exit pipe to prevent the particles from leaving the vessel. The reactor is used to carried out a 98% conversion of a substrate entering at 0.1 kmol m3 ⁄ . Michaelis-Menten kinetics apply, with the following enzyme characteristics: Turnover number = 1 × 10^3 s KM = 5 × 10−3 kmol m3 ⁄ Enzyme RMM = 65,000 (a) If the enzyme is stable, calculate the mass of enzyme that needs to be immobilised. (b) If the enzyme is not stable and experiences exponential denaturation with a half-life of 10 h at the reaction temperature, determine the denaturation constant. (c) How would you expect denaturation to affect the reactor performance?
List the ingredients and recipe (in gram or milligram) to make one liter (1000 ml) of Trypticase Soy Agar, nutrient agar, and Columbia blood medium (use a table
Determine the minimum inhibitory concentrations (MIC) for compounds 1, 2, and 3 based on the microdilution results.
25) In biomaterials design enginnering the surface properties effect the biological interactions (TRUE/FALSE) Chemotaxis Production of ECM Migration of the cells Composition of ECM Responsiveness to extracellular signaling 26) The principal functions of the ECM are ; EXCEPT a) Mechanical support for cell anchorage b) Cell proliferation c) Regulates and determine cells dynamic behaviour: d) Scaffolding for orderly tissue renewal
The effect of an inhibitor I on the rate of a single-substrate, enzyme-catalyzed reaction was investigated and gave the following results (See table below). a. What is the mode of action (pure competitive, mixed competitive, uncompetitive or noncompetitive) of the inhibitor? Briefly explain your answer. b. Estimate the value of Km and Vmax for uninhibited reaction.
Provide images for the discussed solution about the types c and d mechanisms.
Component#, Color, Distance component traveled (mm) Distance solvent front traveled (mm), Rf, Possible identity of component 000 Beta-carotene Chlorophyll a Chlorophyll b xanthophylls Figure 8. TLC chromatogram of the pigments (components) in spinach (example/reference). MS Figure 18. TLC of experimental spinach reference standard (1st, 3rd, and 5th columns) and two unknown plant leaves (2nd and 4th columns). MS Activity #5 (2 pts) Complete Table 5, Components of Unknown Plant Leaves. Use Figure 18 (above) to calculate the Rr value for each component in unknown leaf samples 1 and 2 (columns 2 and 4) using the method described above. Assign a possible identity of each pigment in leaf samples 1 and 2 (columns 2 and 4) based on comparison to the spinach reference standard. If the leaf samples contain pigments or spots that do not have comparable spots in the spinach standard, label these components as "unknown." Table 5. Components of Unknown Plant Leaves RKJC MS RKJC F Component# Color…
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