Component#, Color, Distance component traveled (mm) Distance solvent front traveled (mm), Rf, Possible identity of component 000 Beta-carotene Chlorophyll a Chlorophyll b xanthophylls Figure 8. TLC chromatogram of the pigments (components) in spinach (example/reference). MS Figure 18. TLC of experimental spinach reference standard (1st, 3rd, and 5th columns) and two unknown plant leaves (2nd and 4th columns). MS Activity #5 (2 pts) Complete Table 5, Components of Unknown Plant Leaves. Use Figure 18 (above) to calculate the Rr value for each component in unknown leaf samples 1 and 2 (columns 2 and 4) using the method described above. Assign a possible identity of each pigment in leaf samples 1 and 2 (columns 2 and 4) based on comparison to the spinach reference standard. If the leaf samples contain pigments or spots that do not have comparable spots in the spinach standard, label these components as "unknown." Table 5. Components of Unknown Plant Leaves RKJC MS RKJC F Component# Color Distance component traveled (mm) Distance solvent front Rf traveled (mm) Possible identity of component MS

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Component#, Color, Distance component traveled (mm) Distance solvent front traveled (mm), Rf, Possible identity
of component
000
Beta-carotene
Chlorophyll a
Chlorophyll b
xanthophylls
Figure 8. TLC chromatogram of the pigments
(components) in spinach (example/reference).
MS
Figure 18. TLC of experimental spinach reference standard
(1st, 3rd, and 5th columns) and two unknown plant leaves (2nd
and 4th columns).
MS
Activity #5 (2 pts) Complete Table 5, Components of Unknown Plant Leaves.
Use Figure 18 (above) to calculate the Rr value for each component in unknown leaf samples 1 and 2
(columns 2 and 4) using the method described above. Assign a possible identity of each pigment in leaf
samples 1 and 2 (columns 2 and 4) based on comparison to the spinach reference standard. If the leaf
samples contain pigments or spots that do not have comparable spots in the spinach standard, label these
components as "unknown."
Table 5. Components of Unknown Plant Leaves
RKJC
MS
RKJC
F
Component# Color
Distance component
traveled (mm)
Distance solvent front
Rf
traveled (mm)
Possible
identity
of component
MS
Transcribed Image Text:Component#, Color, Distance component traveled (mm) Distance solvent front traveled (mm), Rf, Possible identity of component 000 Beta-carotene Chlorophyll a Chlorophyll b xanthophylls Figure 8. TLC chromatogram of the pigments (components) in spinach (example/reference). MS Figure 18. TLC of experimental spinach reference standard (1st, 3rd, and 5th columns) and two unknown plant leaves (2nd and 4th columns). MS Activity #5 (2 pts) Complete Table 5, Components of Unknown Plant Leaves. Use Figure 18 (above) to calculate the Rr value for each component in unknown leaf samples 1 and 2 (columns 2 and 4) using the method described above. Assign a possible identity of each pigment in leaf samples 1 and 2 (columns 2 and 4) based on comparison to the spinach reference standard. If the leaf samples contain pigments or spots that do not have comparable spots in the spinach standard, label these components as "unknown." Table 5. Components of Unknown Plant Leaves RKJC MS RKJC F Component# Color Distance component traveled (mm) Distance solvent front Rf traveled (mm) Possible identity of component MS
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