Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. A.You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. B.Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons. C.The read alignment and contig assembly steps would probably be harder for whole genome sequencing. D.The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. E.NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. F.Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.

The Illumina sequencing chemistry is extremely precise, with the great majority of bases scoring Q30…

Answered: Check all the statements that are TRUE…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing.

	A.	You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing.
	B.	Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons.
	C.	The read alignment and contig assembly steps would probably be harder for whole genome sequencing.
	D.	The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer.
	E.	NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency.
	F.	Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.

Expert Solution

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions