Cellulase is an enzyme that breaks down cellulose in the digestive system of some insects such as termites, making glucose available. A team of scientists linked to innovation and development of a multinational company are studying the application to wood of a new product whose active component is extracted from fermented tea leaves, corresponding to a polyphenol that is very stable at changes in pH and temperature. A cellulase enzyme studied in a laboratory acts on the substrate (cellulose) releasing product (glucose). If the substance under study (polyphenol obtained from fermented tea leaves) is added to the medium, the enzyme activity (speed) decreases close to zero, not recovering with increasing substrate concentrations. However, if the test substance is removed from the reaction mixture (by dialysis filtration), the enzyme activity is restored. a) Give a reasoned explanation of the facts (what type of inhibition exerts the polyphenol) b) How this same situation would be observed in a Lineweaver and Burk graph, with and without polyphenol and which kinetic parameter is affected
Cellulase is an enzyme that breaks down cellulose in the
a) Give a reasoned explanation of the facts (what type of inhibition exerts the polyphenol)
b) How this same situation would be observed in a Lineweaver and Burk graph, with and without polyphenol and which kinetic parameter is affected
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