Calculate the transformation efficiency for 105 transformants per ug of DNA if the final recovery volume of the cells was 0.50 ml and the volume plated is 0.10 ml. The quantity of DNA used was approximately 0.025 ug. Determine the transformation efficiency using the formula: Number of transformants ug of DNA O 2,100 O 6,600 O 4.400 8,800 O 88,000 X final vol at recovery (ml) vol plated (ml) = Number of transformants per ug
Calculate the transformation efficiency for 105 transformants per ug of DNA if the final recovery volume of the cells was 0.50 ml and the volume plated is 0.10 ml. The quantity of DNA used was approximately 0.025 ug. Determine the transformation efficiency using the formula: Number of transformants ug of DNA O 2,100 O 6,600 O 4.400 8,800 O 88,000 X final vol at recovery (ml) vol plated (ml) = Number of transformants per ug
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Calculate the transformation efficiency for 105 transformants per ug of DNA if the final recovery volume of the cells was 0.50 ml and the volume plated
is 0.10 ml. The quantity of DNA used was approximately 0.025 ug. Determine the transformation efficiency using the formula:
Number of
transformants
ug of DNA
O2,100
O 6,600
Ⓒ4,400
O 8,800
88,000
O21.000
X
final vol at
recovery (ml)
vol plated (ml)
=
Number of
transformants
per ug
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