Calculate the melting temperature (TM) for the GFP primer CATGGTCCTGCTGGAGTTCGTG. Answer to 2 significant figures.
Q: What is the APC payment for HCPCS code 71046? Explain.
A: APC weight =1.56 Conversion factor CF $80.79 Wage Index 0.9445
Q: What is the purpose of sheep red blood cells in the Paul Bunnell Davidsohn test?
A: Paul Bunnell Davidsohn test is used to screen infectious mononucleosis. It is caused by Epstein Barr…
Q: In Griffith's experiment, the S type bacteria are harmless, but can be transformed into the…
A: In Griffith's experiment, there was a mix of heat-killed S-strain bacteria with live, harmless…
Q: Antisera used in blood typing contain: A. both agglutinins and agglutinogens B. agglutinins…
A: Blood typing is a test performed to determine the blood group. It is also known as ABO typing. In…
Q: Based on the principles of RP-HPLC, which sub fraction in the figure (P1 to P8) is the most polar?…
A: HPLC (High Performance Liquid Chromatography): This method is used for protein purification. Here…
Q: Where is GFP localized within a cell and why?
A: The aggregation of a protein at a given site is known as protein localization. For instance,…
Q: 168cm and 57kg. What is the BMI?
A: The BMI is defined as the body mass index of a person and is found by dividing a person's weight in…
Q: If tryptophan is in high levels, will transcripition stop or initiate?
A: Transcription is a process in which a particular segment of DNA is copied into RNA by the enzyme RNA…
Q: What types of environments limit the application of GFP?
A: The green fluorescent protein (GFP) is used in various microbial applications. However, the GFP…
Q: Show the chemistry involved in conjugating the drug (Calicheamicin-SH) to the antibody in Gemtuzumab…
A: mAb (monoclonal antibody) are antibodies which have monovalent affinity i.e., binding only one…
Q: If you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150…
A: The number of moles of a solute present in one liter of the solution is called the molarity of that…
Q: Describe in detail how a tryptophan attenuator iscontrolled
A: Step 1 A repressible operon system is a positively regulated segment of genetic material which…
Q: In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the…
A: The novel Coronavirus infection SARS CoV 2 can be diagnosed by different means. Proper diagnosis,…
Q: What are the features of the iRobot Ava 500?
A:
Q: Rarely, the conjugation of Hfr and F− cells produces two Hfr cells. Explain how this event takes…
A: The conjugation is the process of transfer of DNA from one donor bacterium to a recipient bacterium.…
Q: 2) Now you would like to raise antibodies to this protein of YFG. How could you use the pure protein…
A: Antibodies generate by the immune system in body against the antigen which is foreign particle…
Q: Discuss the concept of microwave fixation and cryofixation, and why it is suitable for…
A: Microwave fixation and cryofixation are the bilogical techniques which are used for stabilising th⁶e…
Q: Why are platelets not considered cells? And why does it contain a kernel?
A: Platelets, or thrombocytes, are tiny, colorless cell particles in our blood that form clots and hold…
Q: To understand the genes responsible for growth and infectivity in a disease-causing bacterial…
A: Site directed mutagenesis is technique in which Dna is altered and the altered gene product is used…
Q: rom the MW analysis; are any of the gel and immune-detected bands consistent with what you expect…
A: please post questions separately. Answer 1: From the MW Analysis : any of the gel and immune…
Q: PBR327 is descended from PBR322. Explain TWO (2) advantages of using PBR327.
A: pBR327 vectors are created by deleting nucleotides between 1427 and 2516 from pBR322. Those…
Q: Mice and humans with inactivating mutations in the gene encoding activation-induced cytidine…
A: IgM is described as a class of immunoglobulin. It displays its function by serving as the first line…
Q: What will happen to the H antigen precursor if an individual has both A andB genes? Keep in mind…
A: it is found in our blood group.
Q: Why does an Hfr * F- mating not yield two Hfr cells?
A: Conjugation is the process of genetic material transfer between two bacterial cells through direct…
Q: the Salk vaccine require a booster?
A: The poliomyelitis virus causes Polio. The virus infects the nerve cells in the spinal cord, thereby…
Q: Define some physiological alteration of the F factor ?
A: F-factor is the factor associated with a fibre-rich and protein-rich diet. It is an approach to a…
Q: lysozyme was purified through G50 beads. From your lab experience, you can determine that when going…
A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…
Q: When a mixture of different IgG antibody proteins are treated with the enzyme papain, each antibody…
A: Antibodies are immunoglobulin (Ig) glycoproteins generated by plasma cells (B cells) in response to…
Q: Provide a description of the events in each transition for the F factor: a. F- to F+ b. F+ to Hfr…
A: Hey, since there are multiple questions posted, only first question is answered. If you want any…
Q: What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle…
A: The purpose of Southern blotting technique is to identify specific DNA from the mixture of other…
Q: How does the increased Ca21 concentration elicit the cells’ responses?
A: Those compounds that are present in the food that living beings intake for a better and healthy life…
Q: If you wanted to test the effect of a 1:2000 dilution (2,000-fold) of lemon juice on PtK2 cells, how…
A: The formula for calculating a dilution is (C1) (V1) = (C2) (V2) where,1/ C1 is the concentration of…
Q: What will be the preferred mechanism of transportation to ER by the proteins associated with RER?
A: The endoplasmic reticulum is the largest organelle of the cell, extending its network of membrane…
Q: TPA protein function. Use at least two sentences to describe the function of the TPA protein.
A: BASIC INFORMATION BIOMOLECULES These are the molecules that are made up of the biological elements…
Q: What are BAC vectors?
A: Cloning vectors are the vehicles that are used in carrying the foreign DNA molecules. The cloning…
Q: How is “RFLP” technology used in the detection of “SNP” in sickle cell anemia? Please describe the…
A: RFLP is a technique used in molecular biology to detect differences in DNA sequences. The acronym…
Q: What is the excitation and emission maxima of EGFP and how could you use these properties and an…
A: GFP (stands for Green Fluorescent Protein) is a protein that exhibits bright green fluorescence when…
Q: How can you change the concentration of affinity for polymerase to maximize the production and…
A: Answer :- Improvements in protein articulation can be accomplished through lessening the pace of…
Q: In the more common protocol for immunofluorescence detection of cellular proteins, an investigator…
A: Antibodies have Fc and Fab parts. The Fab part binds with the epitope of the antigen. The Fc…
Q: What is an Hfr cell? How does it differ from F-, F+, and F-? What is the definition of an…
A: The bacterial cells carrying the F plasmid are called F+ and those lacking the F plasmid are called…
Q: How would you have to modify the diagram of IgG to depict an IgA molecule isolated from saliva?
A: The B-cells produce five different types of immunoglobulins according to antigens responses. The…
Q: hypothetically speaking, if a rapid test was being made to detect RSV (RSV antigen), the conjugate…
A: introduction Rapid diagnostic test detect presence of viral proteins . Expressed by the covid -19…
Q: Explain what is the agglutination reaction: WHAT agglutinates with what? Agglutination is the…
A: Agglutination reaction is related with the blood group of humans. Blood type in humans is broadly…
Q: In determing Rh group, do you consider the control tube for reading when it shows an agglutination…
A: The system of the Rh blood group is used to classify the blood groups depending on the existence or…
Q: What does BCR ABL positive mean?
A: The cancer of the white blood cells is known as Chronic myelogenous leukemia (CML) in medical terms.…
Q: Provide a description of the events in each transition for the F factor: d. Hfr to F'
A: The transfer of DNA from one bacterium to another through direct contact is called conjugation.
Q: antibodies were produced in a rabbit against a 25 KDa human soluble protien. when the rabbit…
A: In western blotting, the target protein is recognized by a synthetic antibody, called a primary…
Q: 1. 3 4 64 7 10 250 250 150 150 100 100 75 75 Heavy Chain 50 50 37 37 Light Chain 25 25 Std 5.
A: SDS-PAGE is a technique for separation of proteins based on size. Antibody purification is done by…
Q: Why do you suppose that the influenza virus protein that binds the virus to an infected cell is…
A: Hemagglutinin are glycoproteins which cause red blood cells to agglutinate or clump together.
Calculate the melting temperature (TM) for the GFP primer CATGGTCCTGCTGGAGTTCGTG. Answer to 2 significant figures.
.....
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Regarding the production of recombinant monoclonal antibodies, it is CORRECT to state that: * (JUST ONE STATEMENT IS CORRECT) (A) Chimeric and humanized antibodies differ in relation to the percentage of sequence of the constant chain of molecules from the human genome (B) Chimeric antibodies are industrially produced by joining purified protein fragments of the Fab fraction of murine antibodies and the constant portion of human antibodies. (C) When using cells dependent on the DHF amplification system, we must use a vector containing the coding information of the light and heavy chains and another vector that contains the coding sequence of the enzyme dihydrofolate reductase (D)The industrial purification of monoclonal antibodies should preferably use an initial step based on the use of affinity columns, which will bind the antibodies by the Fc fraction (E) The use of amplification systems such as GS and DHFR has as main objective to make the production of monoclonal antibodies…Given below is the electrophoretic profile of 2 proteins, a normal hemoglobin, HbA and the fetal hemoglobin, HbF. What information can be obtained from the profile shown? wwwwwww (+) HbF ww w (-) HbA ww ww A. HbF has a slightly different conformation compared with HbA B. HbF and HbA have different primary structures C. HbF has a higher affinity for oxygen than HbA D. HbF has a nonpolar amino acid residue in place of a basic amino acid. E. HbF has an acidic amino acid residue in place of a nonpolar amino acidHuman serum transferrin is an iron transport protein. With the use of appropriate diagrams, describe the metal-bound active site and discuss the structural changes that occur on iron binding to the protein.
- The anticlotting property of heparin is partly the result of the negative charges it carries. ( Q.) Which type of heparin is a better anticoagulant, one with a high or a low degree of polymerization?After purifying the expressed protien His6-NEP of interest, the different fractions collected will be run on an SDS-Page gels. If our purification was successful a band of correct size should be present in: A) orginal lysate B) lysate flowthrough C) wash flowthrough D) Elute E) orginal lystae and elite fractionsWhen a mixture of different IgG antibody proteins are treated with the enzyme papain, each antibody is cleaved into three roughly equal size fragments. From each original antibody, two of the three fragments are identical to each other, and represent the ‘arms’ of the antibody ‘Y’. These fragments are known as Fab fragments. The third fragment is known as the Fc region, because this fragment will crystallize when purified. The reason a mixture of Fc fragments will crystallize is because: It is the only part of the antibody protein that can easily be purified at the high concentrations needed for crystallization. It has no disulfide bonds holding the domains together, as disulfide bonds will inhibit crystallization. It is the only fragment of the antibody that still has disulfide bonds, so it remains intact during the crystallization process. The Fc fragments of IgG are much more water soluble than the Fab fragments. All Fc fragments generated from a mixture of IgG molecules have the…
- A) 43 14 When Friend erythroleukemic cells are incubated with sodium butyrate, they differentiate into nondividing, hemoglobin-synthesizing cells. Butyrate-induced differentiation is accompanied by accumulation of acety- lated forms of H3 and H4 histones, as can be seen most clearly for H4 in Fig- ure 4-16A. In principle, butyrate treatment could increase the activity of his- tone acetyl transferases (HATS) or decrease the activity of histone deacety- lase complexes (HDACs). To distinguish between these alternatives, HAT and HDAC activities were measured in the presence and absence of butyrate (Figure 4-16B and C). From these results decide how butyrate treatment causes accumulation of acetylated histones. butyrate (B) H-acetylated histone (cpm, thousands) N -butyrate + butyrate boiled 9 30 10 20 time (minutes) (C) C free 'H-acetate (cpm, thousands) S43210 boiled -butyrate +butyrate,Give typed explanation Which of the following effects is NOT true? If stripped Hb is treated with 2,3-BPG, the O2-binding curve for Hb will shift left. If stripped Hb is treated with 2,3-BPG and CO2, the O2-binding curve for Hb will mirror that of fully intact Hb. If stripped Hb is treated with CO2, the O2-binding curve for Hb will shift right. All of the listed effects are true.Two co-op students at your start-up company have been asked to evaluate the rate of reaction occurring in a transparent gel particle containing immobilized mouse melanoma cells. The equation for the reaction rate ris is: Vmax T'As Km+CAs where R=3.2 mm, Vmax= 0.12 gmol s' m3, CAS = 41 gmol m3, and Km=0.8 gmol m3. One student reports a reaction rate of 1.6x10 gmol st; the other reports 1.6x10 1º gmol s1. You left your calculator on the bus this morning, but must know quickly which student is correct. Use an order-of- magnitude calculation to identify the right answer. Which student is correct?
- (e) Two ADP agonists (drugs) were also found to bind to platelets: 2-methylthio-ADP bound with a Kp of 7 µM and 2-(3-aminopropylthio)-ADP bound with a Kp of 200 µM. Can these drugs effectively compete with ADP for binding to platelets? Explain your answer.r^2=6Dt , where D is the diffusion coefficient of thediffusing object and t is the time that the object is allowed to diffuse. If the diffusion coefficient, D, of a small protein is 5 x 10^-10 m2 s-1, how long (on average) does ittake for the protein to diffuse across a parasitic wasp that is 189 um long ?please help me I can't find answers for these questions: here is the link for the article https://www.pbs.org/wgbh/nova/transcripts/2805cancer.html What type of substances are angiostatin and endostatin and where are they produced? What do they do? A) describe the experiments using cow bones to discover anti-angiogenic substances. Why was this used as a source of these potential proteins? B) describe the “accidental” discovery of a novel antiangiogenic substance because of lab contamination?