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c. In lab, you recorded a UV-Vis spectrum of a mixture of proteins. What best explains why two major peaks were observed in your spectrum between 240 and 480 nm? i. Amino acids absorb light at two wavelengths depending on their identity. ii. iii. iv. The sample was impure. All proteins absorb light at 280 due to the presence of aromatic amino acids and red proteins absorb light near 400 nm. The buffer absorbs light in the visible region and the proteins absorb light in the UV region of the spectrum.

c. In lab, you recorded a UV-Vis spectrum of a mixture of proteins. What best explains why two major peaks were observed in your spectrum between 240 and 480 nm? i. Amino acids absorb light at two wavelengths depending on their identity. ii. iii. iv. The sample was impure. All proteins absorb light at 280 due to the presence of aromatic amino acids and red proteins absorb light near 400 nm. The buffer absorbs light in the visible region and the proteins absorb light in the UV region of the spectrum.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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c. In lab, you recorded a UV-Vis spectrum of a mixture of proteins. What best explains why two major peaks were observed in your spectrum between 240 and 480 nm? i. Amino acids absorb light at two wavelengths depending on their identity. The sample was impure. All proteins absorb light at 280 due to the presence of aromatic amino acids and red proteins absorb light near 400 nm. The buffer absorbs light in the visible region and the proteins absorb light in the UV region of the spectrum. None of the above. ii. iii. iv. V. d. In lab, you ran an SDS-PAGE gel. What best explains the importance of adding SDS and heating your samples? i. SDS is a dye used for visualization and heat is used to dissolve the SDS in solution. iii. iv. SDS coats the protein with negative charge and heat aids in the unfolding of the proteins. SDS and heat both help stabilize the proteins during gel electrophoresis. SDS and heat both help break the peptide bonds of proteins, which is necessary for gel electrophoresis.
Transcribed Image Text:c. In lab, you recorded a UV-Vis spectrum of a mixture of proteins. What best explains why two major peaks were observed in your spectrum between 240 and 480 nm? i. Amino acids absorb light at two wavelengths depending on their identity. The sample was impure. All proteins absorb light at 280 due to the presence of aromatic amino acids and red proteins absorb light near 400 nm. The buffer absorbs light in the visible region and the proteins absorb light in the UV region of the spectrum. None of the above. ii. iii. iv. V. d. In lab, you ran an SDS-PAGE gel. What best explains the importance of adding SDS and heating your samples? i. SDS is a dye used for visualization and heat is used to dissolve the SDS in solution. iii. iv. SDS coats the protein with negative charge and heat aids in the unfolding of the proteins. SDS and heat both help stabilize the proteins during gel electrophoresis. SDS and heat both help break the peptide bonds of proteins, which is necessary for gel electrophoresis.
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