C G C T A T с T T T C C G G rite the nucleotide sequence of the newly synthesized DNA, without the primer, in the 3'to 5' direction.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide
triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at
their characteristic wavelengths. An automated machine then reads the sequence.
In this procedure,
• ddCTP is tagged with a blue marker that emits light at 517 nm,
• ddATP is tagged with a green marker that emits light at 540 nm,
•
ddGTP is tagged with a yellow marker that emits light at 570 nm, and
ddTTP is tagged with a red marker that emits light at 635 nm.
The primer has the sequence 5'-ATGAC-3'. In the image shown here, the shortest fragments are at the bottom and the
longest fragments are at the top.
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Write the nucleotide sequence of the newly synthesized DNA, without the primer, in the 3'to 5' direction.
31-
5
Transcribed Image Text:A researcher sequences a DNA fragment using dye-terminator sequencing. In this procedure, each dideoxynucleotide triphosphate (ddNTP) contains a fluorescent marker specific to the base. Upon excitement by a laser, the ddNTPs emit light at their characteristic wavelengths. An automated machine then reads the sequence. In this procedure, • ddCTP is tagged with a blue marker that emits light at 517 nm, • ddATP is tagged with a green marker that emits light at 540 nm, • ddGTP is tagged with a yellow marker that emits light at 570 nm, and ddTTP is tagged with a red marker that emits light at 635 nm. The primer has the sequence 5'-ATGAC-3'. In the image shown here, the shortest fragments are at the bottom and the longest fragments are at the top. с G с T A T T T T T с G G Write the nucleotide sequence of the newly synthesized DNA, without the primer, in the 3'to 5' direction. 31- 5
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