Answer the following 2 questions based on the given sequence of DNA. The top strand is the template strand: 5'-CGTATAGCTCAGGGCGCTAACCTTAGCATGGACG-3' (Template) 3'-GCATATCGAGTCCCGCGATTGGAATCGTACCTGC-5' (Coding) 1. Write the mRNA sequence that will be produced when this DNA segment is transcribed. 2. Write the amino acid sequence that will be produced when this segment is translated.

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Answer the following 2 questions based on the given sequence of DNA.  The top strand is the template strand:

 

5’-CGTATAGCTCAGGGCGCTAACCTTAGCATGGACG-3’ (Template)

3’-GCATATCGAGTCCCGCGATTGGAATCGTACCTGC-5’ (Coding)

**DNA Transcription and Translation: An Interactive Exercise**

Explore the principles of DNA transcription and translation using the following exercise.

### DNA Sequence Analysis

Given the following DNA sequence, where the top strand serves as the template:
- **Template Strand (3’ to 5’):** 5’-CGTATAGCTCAGGCGGCTAACC
TTAGCATTGACG-3’
- **Coding Strand (5’ to 3’):** 3’-GCATATCGAGTCCGCCGATTG
GAATCGTACCTGC-5’

### Tasks:

1. **Transcription: mRNA Sequence**
   - Transcribe the given DNA template strand into the mRNA sequence.

2. **Translation: Amino Acid Sequence**
   - Using the mRNA codon chart provided, translate the mRNA sequence to its corresponding amino acid sequence.

### mRNA Codon Chart Explanation

The chart shown illustrates the genetic code, defining how codons specify amino acids. It is organized by the first, second, and third nucleotide positions of the codons.

- **First Position:** Located on the left side, with options U, C, A, G.
- **Second Position:** Showcased on the top, creating specific matrices for U, C, A, G.
- **Third Position:** Detailed within each individual box, again with U, C, A, G.

#### Example Codon Usage:

- **AUG:** Represents Methionine (start codon).
- **UAA, UAG, UGA:** Represent stop codons.

### Answers:

1. **mRNA Sequence:**
   - Transcribed from the template strand, the mRNA sequence will substitute thymine (T) with uracil (U).

2. **Amino Acid Sequence:**
   - Using the mRNA codon, refer to the chart to determine the sequence of amino acids that will be formed during translation.

This approach aids in understanding how genetic information encoded in DNA is utilized to produce proteins, a fundamental process in molecular biology.
Transcribed Image Text:**DNA Transcription and Translation: An Interactive Exercise** Explore the principles of DNA transcription and translation using the following exercise. ### DNA Sequence Analysis Given the following DNA sequence, where the top strand serves as the template: - **Template Strand (3’ to 5’):** 5’-CGTATAGCTCAGGCGGCTAACC TTAGCATTGACG-3’ - **Coding Strand (5’ to 3’):** 3’-GCATATCGAGTCCGCCGATTG GAATCGTACCTGC-5’ ### Tasks: 1. **Transcription: mRNA Sequence** - Transcribe the given DNA template strand into the mRNA sequence. 2. **Translation: Amino Acid Sequence** - Using the mRNA codon chart provided, translate the mRNA sequence to its corresponding amino acid sequence. ### mRNA Codon Chart Explanation The chart shown illustrates the genetic code, defining how codons specify amino acids. It is organized by the first, second, and third nucleotide positions of the codons. - **First Position:** Located on the left side, with options U, C, A, G. - **Second Position:** Showcased on the top, creating specific matrices for U, C, A, G. - **Third Position:** Detailed within each individual box, again with U, C, A, G. #### Example Codon Usage: - **AUG:** Represents Methionine (start codon). - **UAA, UAG, UGA:** Represent stop codons. ### Answers: 1. **mRNA Sequence:** - Transcribed from the template strand, the mRNA sequence will substitute thymine (T) with uracil (U). 2. **Amino Acid Sequence:** - Using the mRNA codon, refer to the chart to determine the sequence of amino acids that will be formed during translation. This approach aids in understanding how genetic information encoded in DNA is utilized to produce proteins, a fundamental process in molecular biology.
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