Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M mannitol. What was the purpose of including mannitol at this concentration?

Q: Why is GAPDH not be a suitable loading control for nuclear extracts?

A: * In many molecular biology assays, such as Western blotting, the housekeeping gene glyceraldehyde…

Q: What is differential centrifugation? Explain how it can be used to separate cellular organelles.

A: Differential centrifugation : A separation method where the components of a cell are separated on…

Q: What are the different categories of cell viability assays? Describe one of them briefly. Define the…

A: Cell viability assays are the assays which are mainly used to determine or find out the number of…

Q: BACTERIA STRAIN A IS AUXOTROPHIC FOR METHIONINE AND STRAIN B IS AUXOTROPHIC FOR LEUCINE. A. WILL…

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Q: Suppose you have a cell-lysis sample containing genomic DNA and a complex mixture of proteins and…

A: DNA is the genetic material of most cells. It is made of nucleotides, which are composed of a…

Q: "the supplement that restored growth would be the molecule that the mutant strain could not…

A: Introduction: Mutant strain grew on insignificant medium with amino acids, mutants should not have…

Q: What is the attached enzyme in this assay and its corresponding substrate? A. Horseradish peroxidase…

A: INTRODUCTION ELISA Enzyme linked Immunosorbent assay, is a technique used to detect antibodies in…

Q: You generate several temperature sensitive mutant strains of bacteria. To study what genes might be…

A: Introduction :- Bacterial mutants frequently lose a growth characteristic (such as the inability to…

Q: Using the plasmid map attached and the answer from the previous question, draw a simplified diagram…

A: The image you provided shows a diagram of the YCplac33 plasmid. This plasmid is a commonly used…

Q: Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some…

A: The melting curves are generated when the DNA is heated along with the fluorescent DNA-binding dye.…

Q: In the Avery, McCarty and McCleod experiment, DNA was identified as the heritable material because,…

A: A long time back it was unknown which the genetic material among protein, RNA, and DNA. It was later…

Q: How did the Hershey–Chase “blender experiments” confirmGriffith’s results?

A: Based on the Griffith experiment's observations, he concluded that genetic material is the…

Q: Explain the purpose of the Kirby-Bauer diffusion test?

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Q: Why is it essential to add sodium azide to succinate dehydrogenase assay  solution? What is the…

A: In laboratories various assays are carried out for the determination of functionality of various…

Q: In

A: STR typing or short tendum repeat analysis is a molecular biology technique used to study or analyze…

Q: Is the yeast strain HA0-1 complementary to HB1 or HB2?Explain.

A: Complementation analysis is generally analysed to identify the mutations in yeast through adenine…

Q: What is the formula for transformation efficiency?

A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…

Q: Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M…

A: Introduction  The study of fundamental processes, such as mitochondrial respiration, metabolic…

Q: What is the function of the chaotropic salt in DNA isolation?

A: These salts are strong protein denaturing agents.

Q: What is the purpose of adding two drops of acetic acid in thin layer chromatography of plant…

A: Plant pigments are natural substances found in plant cells that give color to different parts of the…

Q: A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a…

A: The inventors of fluctuation test were Delburk and Luria in 1943. This test aimed to determine…

Q: Explain how the use of radiolabeled amino acids in this procedure helped to reveal the genetic code.

A: The nucleotide sequence in the genetic material which recognizes the amino acid sequence is called…

Q: Two auxotrophic triple Escherichia coli strains (A: met- phe- ade- val+ bio+ thr+ and B: met+ phe+…

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Q: A bloactlve hexapeptide was cleaved by trypsln from a larger proteln. The peptlde was sequenced by…

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Q: What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each…

A: The DNA (deoxyribonucleic acid) is the hereditary nucleic acid sequence that contains the genetic…

Q: Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: Hyperchromicity is the increase in optical density of a substance at a particular wavelength.

Q: why are 2D NMR spectra symmetrical

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Q: polarimetry what happens to the extent of inversion when the sugar is heated longer than the…

A: Polarimetry is a analytical tool used to measure the concentration of a substance in a solution  Its…

Q: You grow E. coli in defined media containing both glucose and lactose. Draw the trajectory of the…

A: The lac operon It is a set of three genes in E.coli that code for enzymes that help the cell use…

Q: The GC content of the DNA in the genome of a newly discovered bacterium is 46%, what is the…

A: Chargaff’s rules state that the DNA content from any cell of any organism should always have a 1:1…

Q: CPT code assignment 44970

A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…

Q: As you are performing this protocol, you realize that the ultraviolet (UV) light does not work as…

A: Introduction A gene is a DNA segment that encodes for protein or RNA (ribonucleic acid). It is the…

Q: Suppose you want to figure out what genes are involved in the production of proline (Pro) in a…

A: Introduction :- Proline is one of the  essential amino acid .It is the only proteinogenic secondary…

Q: What is the best amount of concentration of DAPI II for counterstaining human chromosomes?

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Q: I am currently using Molecular imprinted polymer(MIP) to extract oleic acid from Palm Fatty Acid…

A: Solid phase extraction (SPE) is a technique in which compounds that are dissolved in a liquid…

Q: What is the purpose of a standard curve for a colorimetric enzyme assay?

A: Enzyme assays are mostly used to determine the vitamin status in the body. The activity of…

Q: Both the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M…

A: Mitochondria is a double membrane organelle with a rod like shape. It produces chemical energy in…

Q: What are the reaction bat the molecular level of denaturation of frying egg. Explain step by step…

A: Egg is a good dietary source of protein. Egg also contain mono and polyunsaturated fatty acids,…

Q: Why does the nucleus appear deep blue after staining with methylene blue? Is it possible that…

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Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: Galactosidase enzyme is considered a better selectable marker. Justify the statement.

A: Enzymes are proteins that enable our bodies' metabolism, or chemical reactions, go more quickly.…

Q: Give an example of a scenario in which you could partially isolate a protein with differential…

A: To find out the amount of ammonium sulfate to the added to a protein solution to precipitate the…

Food Microbiology

The subdivision of microbiology that deals with the interaction between microbes and food particles are called food microbiology. This scientific study incorporates both beneficial and harmful microbes and their effect on the food production, food storage, and health of human beings.