Biotechnology – Complete the problems below regarding RE and Gel Electrophoresis. The following DNA sequence is from a virus that is dangerous, scientists want to use a restriction enzyme to cut the virus into bits. They do not need sticky ends because the do not plan to combine it with other DNA. Use HindII to show how this DNA would be cut. HindII GTC/GAC CAG/CTG Use HindIII to cut the DNA strand below. Use / to show the cut sites. G A A A A G T C G A C A A G G C A G T C G A C T T T T A A A A G T C G A C A T G C C T T T T C A G C T G T T C C G T C A G CT G A A AA T T T T C A G C T G T A C G
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
-
Biotechnology – Complete the problems below regarding RE and Gel Electrophoresis.
The following DNA sequence is from a virus that is dangerous, scientists want to use a restriction enzyme to cut the virus into bits. They do not need sticky ends because the do not plan to combine it with other DNA. Use HindII to show how this DNA would be cut.
HindII GTC/GAC
CAG/CTG
- Use HindIII to cut the DNA strand below. Use / to show the cut sites.
G A A A A G T C G A C A A G G C A G T C G A C T T T T A A A A G T C G A C A T G C C T T T T C A G C T G T T C C G T C A G CT G A A AA T T T T C A G C T G T A C G
1. How many pieces of DNA would you have?
2. How many base pairs in length will each piece be? (Hint: Count the letters between cuts)
- Put those pieces of DNA into well 1 and then show where they will migrate on the gel during gel electrophoresis. Make sure to draw band and label bp length.
|
Well 1 |
DNA Ladder |
|
|
------- 15 bp |
|
|
|
|
|
------- 13 bp |
|
|
|
|
|
|
|
|
------- 10 bp |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
------- 5 bp |
|
|
|
|
|
|
|
|
|
|
|
------- 1 bp |
|
|
|
|
|
|
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 1 images
