## BiotechnologyThere are several different tools used to study or manipulate DNA. Match the description to the technology.### InstructionsIf you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice.### Technologies and Descriptions1. **Polymerase Chain Reaction (PCR)** - (Description to be selected from the list) 2. **Gel Electrophoresis** - (Description to be selected from the list) 3. **Molecular cloning** - (Description to be selected from the list) 4. **Restriction enzyme** - (Description to be selected from the list) 5. **Plasmids** - (Description to be selected from the list) 6. **CRISPR** - (Description to be selected from the list) 7. **Gene therapy** - (Dropdown menu selection: Replacing defective genes that caused a disease)### Descriptions Available- Circular DNA used to insert genes- Cuts DNA into smaller pieces- Tool for gene editing- Creates larger quantities of DNA- Produces genetically identical DNA fragments- Separates fragments of DNA by size- Replacing defective genes that caused a disease### Interactive FeatureEach technology allows you to choose its matching description from the dropdown menu to enhance your understanding of its function in biotechnology.

The field of biotechnology has evolved enormously over the years. it's gone from the normal read of…

ere are several different tools used to study or manipulate DNA. Match the description to the technology. TE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make erent choice. plymerase Chain Reaction (PCR) Replacing defective genes that caused a disease el Electrophoresis Circular DNA used to insert genes olecular cloning Cuts DNA into smaller pieces Tool for gene editing estriction enzyme Creates larger quantities of DNA asmids Produces genetically identical DNA fragments Separates fragments of DNA by size RISDR

ere are several different tools used to study or manipulate DNA. Match the description to the technology. TE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make erent choice. plymerase Chain Reaction (PCR) Replacing defective genes that caused a disease el Electrophoresis Circular DNA used to insert genes olecular cloning Cuts DNA into smaller pieces Tool for gene editing estriction enzyme Creates larger quantities of DNA asmids Produces genetically identical DNA fragments Separates fragments of DNA by size RISDR

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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which one or more than one shows successful DNA sequencing in mutagenesis.
Steps in PCR and Gene Cloning Propose two hypothetically modified or genetically engineered plants and/or animals [based on your own creative ideas] that can be used for health, industry, agriculture, and environmental protection. Do consider the ethical standards in creating your GMO. Draw parts of the expression vector. List the steps in restriction digestion and PCR.
● Gene lacZ fliE Please fill in the table below for the desired mutations from section five. Please reword any pathway information you find and reference the source (not just ecocyc.com) omp A lexA Size of gene (bp) 3075 315 rpoA nagC 1221 990 1041 609 Protein Pathway Expected size of PCR (bp) name
CRISPR One of the more recent advances in biotechnology is the development of the CRISPR gene modification tool. Match the following descriptions the the key players in this technology. NOTE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice. CRISPR technology Specialized stretches of DNA with nucleotide repeats and Cas9 protein spacers Enzyme that cuts DNA crRNA Guides the enzyme to the target site CRISPR DNA Adapted from the natural defense mechanism of bacteria and archaea
Please help me, this is from practice, double check your answers, previous tutors got it wrong
How would you go about cloning this amplified DNA into pL4440？Using your knowledge of cloning describe 5 important aspects of the method in details
Answer the following: What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from lecture. • Describe the steps of DNA fingerprinting. Include the following terms underlined: o DNA sample o PCR o restriction enzyme o gel electrophoresis If you haven't done so already, explain the steps of PCR in detail - also explain the steps of gel electrophoresis in detail.
Plasmids usually contain a gene for to a make make it easy to grow only colonies of bacteria that have acquired the plasmid. antibiotic resistance O lactose intolerance color O apoptosis
Part A How is a gene for a particular protein inserted into a plasmid? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. phosphate groups sticky ends an apurinic enzyme nitrogen-containing bases a recombinant DNA joined Submit cut ovide Feedback a donor DNA a restriction enzyme Request Answer When a gene has been obtained using Reset Help it is mixed with the plasmids that have been by the same enzyme. When mixed together in a fresh E. coli culture, the of the DNA fragments bond with the of the plasmid DNA to form
In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI
Give answers to the questions in the picture
Place the steps in the correct order from start to finish. Do not overlap steps. Start Transformed plant cells are selected for on a culture medium containing herbicide Bacteria are transformed with two plasmids Transgenes are incorporated into the plant cell genome T-DNA is transferred from the bacterial cell to the plant cell Transformed bacteria conjugate with plant cells Reset Transformed plant cells grow into transgenic plants Help Finish