You are a scientist studying the function of a gene you find interesting. You need to produce protein in E.coli so you can purify it and run for functional assay. Which of the following steps is not part of the process of cloning the gene? Amplifying the target gene through PCR. O Cutting both foreign DNA and plasmid DNA with the same restriction enzyme. Adding DNA ligase to create a hydrogen bond to seal the nick in the backbone. Introducing circular recombinant DNA to host bacterial cells. O Selecting the cells with recombinant DNA using selectable markers.
You are a scientist studying the function of a gene you find interesting. You need to produce protein in E.coli so you can purify it and run for functional assay. Which of the following steps is not part of the process of cloning the gene? Amplifying the target gene through PCR. O Cutting both foreign DNA and plasmid DNA with the same restriction enzyme. Adding DNA ligase to create a hydrogen bond to seal the nick in the backbone. Introducing circular recombinant DNA to host bacterial cells. O Selecting the cells with recombinant DNA using selectable markers.
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 8QP
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Step 1: Importance of protein production
VIEWStep 2: Amplifying the target gene through PCR
VIEWStep 3: Cutting both foreign DNA and plasmid DNA with the same restriction enzyme
VIEWStep 4: Adding DNA ligase to create a hydrogen bond to seal the nick in the backbone
VIEWStep 5: Introducing circular recombinant DNA to host bacterial cells
VIEWStep 6: Selecting the cells with recombinant DNA using selectable markers
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