Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication. **Enzymes and Proteins:** - **Ligase** - **Helicase** - **Primase** - **DNA Polymerase I** - **DNA Polymerase III** - **Bacterial Gyrase** - **SSBP's (Single-Strand Binding Proteins)** **Solutions:** - Using RNA as a template to make DNA - Conversion of double-stranded DNA into single-stranded templates - Synthesis of most of DNA - Replacement of RNA with DNA - Relief of chromosome supercoiling - Synthesis of a short RNA primer - Protection of single-stranded DNA templates - Covalent linkage of Okazaki fragments --- Learners can use the dropdown menus beside each enzyme or protein to match it with the appropriate solution based on its function in DNA replication.

Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication. Enzymes and Proteins: - Ligase - Helicase - Primase - DNA Polymerase I - DNA Polymerase III - Bacterial Gyrase - SSBP's (Single-Strand Binding Proteins) Solutions: - Using RNA as a template to make DNA - Conversion of double-stranded DNA into single-stranded templates - Synthesis of most of DNA - Replacement of RNA with DNA - Relief of chromosome supercoiling - Synthesis of a short RNA primer - Protection of single-stranded DNA templates - Covalent linkage of Okazaki fragments --- Learners can use the dropdown menus beside each enzyme or protein to match it with the appropriate solution based on its function in DNA replication.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Which of the following mechanisms does not contribute to reducing the overall in vivo mutation rate found in most species? Mismatch repair of incorrectly inserted bases Replication-associated proteins, in addition to DNA polymerase, aid in fidelity of replication O Excision repair of thymine dimers O Tautomerization of nitrogenous bases Proofreading by DNA polymerase's 3' to 5' exonuclease activity
An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)
How is DNA copied with leading and lagging strands? Please use these terms in a summary: Helicase Decide on the leading vs lagging strand locations Primase on leading strand DNA polymerase Primase on lagging strand Ligase Nuclease
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The map of plasmid pUC19 is shown below. The restriction site coordinate is the position of the 5’base on the top strand of each site sequence. The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.
You are studying a colony of cells and determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. A) What will the effect of the mutation in DNA polymerase I be on DNA replication? In your answer make sure to describe what would be observed in the leading and lagging strand and explain your reasoning. B) Will this mutation in DNA polymerase I have an impact on another step in DNA replication? In your answer make sure to indicate whether DNA replication will be impacted or not. If it is not, explain why. If it is impacted, then describe the step that is impacted and name the molecule or enzyme involved.
What is/are the attributes that make nucleotide excision repair (NER) and base excision repair (BER) similar and/or different from each other? Select the correct response: The NER pathway is the only one that can remove DNA lesions in the strand regardless of their size which is followed by attaching the correct strand, then sealed by a DNA ligase. They both use the enzyme DNA glycosylases that recognizes the damaged DNA segments and proceed with repairing the faulty base in the strand. They differ NER only repairs purine bases while BER repairs pyrimidine bases. They both remove the damaged parts of the DNA where the BER pathway corrects only the identified damaged bases which are usually non-bulky lesions. The NER pathway, on the other hand, repairs the damage by removal of bulky DNA adducts which is a short-single stranded DNA segment. They both utilize the enzyme photolyase to reverse the damages created by the faulty section of the DNA. They both remove the damaged parts of the…
Below is a list of types of repair systems. Please make sure to select ALL of the processes that can repair double-stranded breaks. base-excision repair O mismatch repair homologous recombination Onucleotide-excision repair ONon-homologous end joining
Which of the following plays a role in repairing thymine dimers by reversing the damage caused by UV light exposure in bacteria? base excision repair photoreactivation enzyme postreplication repair non-homologous end-joining repair DNA polymerase proofreading
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Using the figure below, what is molecule "A" (type a 1, 2 or 3 in the blank) nuclease ligase DNA polymerase What is the function of molecule "A"? to separate the double helix into two to piece together the Okazaki segments to copy the new DNA strand to the old strand by complementary base pairing Using the figure below, what is molecule "G" (type a 1, 2 or 3 in the blank) nuclease ligase DNA polymerase What is the function of molecule "G"? to separate the double helix into two to piece
A cytosine deamination occurs in the top strand of the following DNA duplex 5'-gATTACA-3'3'-CTAATgT-5' The duplex is then replicated before the deamination is repaired. What will be sequence of the newly synthesized bottom strand? Your answer should read 3' to 5'