First start with a pre-MRNA with four exons and three introns and diagram the splicing reactions leading to the four exons being spliced together (a) Second, show the following two alternative splicing diagrams that would produce (b) intron 2 retention, otherwise similar to part (a) (c) mutually exclusive exon (exon 1- either exon 2 or exon 3 -- exon 4) (d) exon 2 skipping, otherwise, similar to part (a). For part (d), explain how an SR protein could influence whether exon 2 is skipped. What would happen if SR binding to the exon 2 ESE was weak? Which MRNA isoform would be more abundant?
First start with a pre-MRNA with four exons and three introns and diagram the splicing reactions leading to the four exons being spliced together (a) Second, show the following two alternative splicing diagrams that would produce (b) intron 2 retention, otherwise similar to part (a) (c) mutually exclusive exon (exon 1- either exon 2 or exon 3 -- exon 4) (d) exon 2 skipping, otherwise, similar to part (a). For part (d), explain how an SR protein could influence whether exon 2 is skipped. What would happen if SR binding to the exon 2 ESE was weak? Which MRNA isoform would be more abundant?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:First start with a pre-MRNA with four exons and three introns and diagram the splicing reactions leading
to the four exons being spliced together (a)
Second, show the following two alternative splicing diagrams that would produce
(b) intron 2 retention, otherwise similar to part (a)
(c) mutually exclusive exon (exon 1- either exon 2 or exon 3 -- exon 4)
(d) exon 2 skipping, otherwise, similar to part (a).
For part (d), explain how an SR protein could influence whether exon 2 is skipped. What would happen if
SR binding to the exon 2 ESE was weak? Which MRNA isoform would be more abundant?
Expert Solution
Alternative splicing
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Alternative splicing of a pre-mRNA is the phenomenon by which different combinations and patterns of exons and introns get incorporated to form mRNA's with different genetic content .
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