Biochemistry techniques: a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection. Focus on antigens and antibodies. Justify your reasoning. B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute last (D) arrangement below: A) B) C) D) Explain your reasoning . Instructor C) . What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS- PAGE?

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