Bicarbonate Buffer a. Prepare 100 mL of 0.200 bicarbonate buffer, pH 10, by calculating the mass of sodium bicarbonate and sodium carbonate. b. Use the Henderson-Hasselbalch equation to calculate the volume of each stock solution needed. pH = pKa + log [conjugate base] / [weak acid]1 c. Check your calculations with other students. See the instructor if there is uncertainty. d. Make the solution and check the pH of a portion of your buffer solution using the pH meter. L = 245μL Preparation of Bicarbonate Buffer Calculated Volume: CH3COOH = _______________ KH2PO4 = ________________ Actual pH of the buffer: _____12.65_____ NOTE: Please answer letter A, I need the volume. I already know how to calculate the actual pH. Thank you.
Bicarbonate Buffer
a. Prepare 100 mL of 0.200 bicarbonate buffer, pH 10, by calculating the mass of sodium bicarbonate and sodium carbonate.
b. Use the Henderson-Hasselbalch equation to calculate the volume of each stock solution needed. pH = pKa + log [conjugate base] / [weak acid]1
c. Check your calculations with other students. See the instructor if there is uncertainty.
d. Make the solution and check the pH of a portion of your buffer solution using the pH meter. L = 245μL
Preparation of Bicarbonate Buffer
Calculated Volume:
CH3COOH = _______________
KH2PO4 = ________________
Actual pH of the buffer: _____12.65_____
NOTE: Please answer letter A, I need the volume. I already know how to calculate the actual pH. Thank you.
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